Viral respiratory infections and the oropharyngeal bacterial microbiota in acutely wheezing children


Autoři: Leah Cuthbertson aff001;  Stephen W. C. Oo aff002;  Michael J. Cox aff001;  Siew-Kim Khoo aff002;  Des W. Cox aff002;  Glenys Chidlow aff005;  Kimberley Franks aff002;  Franciska Prastanti aff002;  Meredith L. Borland aff002;  James E. Gern aff008;  David W. Smith aff002;  Joelene A. Bizzintino aff002;  Ingrid A. Laing aff002;  Peter N. Le Souëf aff002;  Miriam F. Moffatt aff001;  William O. C. Cookson aff001
Působiště autorů: National Heart and Lung Institute, Imperial College, London, England, United Kingdom aff001;  Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia aff002;  Respiratory Department, Perth Children’s Hospital, Perth, Western Australia aff003;  Telethon Kids Institute, Perth, Australia aff004;  Department of Microbiology, PathWest Laboratory Medicine WA, QEII Medical Centre, Perth, Australia aff005;  Emergency Department, Perth Children’s Hospital, Perth, Australia aff006;  Division of Emergency Medicine, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia aff007;  Department of Pediatrics, University of Wisconsin-Madison, Madison, Wisconsin, United States of America aff008;  Medical School, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia aff009;  Royal Brompton and Harefield NHS Foundation Trust, London, England, United Kingdom aff010
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223990

Souhrn

Acute viral wheeze in children is a major cause of hospitalisation and a major risk factor for the development of asthma. However, the role of the respiratory tract microbiome in the development of acute wheeze is unclear. To investigate whether severe wheezing episodes in children are associated with bacterial dysbiosis in the respiratory tract, oropharyngeal swabs were collected from 109 children with acute wheezing attending the only tertiary paediatric hospital in Perth, Australia. The bacterial community from these samples was explored using next generation sequencing and compared to samples from 75 non-wheezing controls. No significant difference in bacterial diversity was observed between samples from those with wheeze and healthy controls. Within the wheezing group, attendance at kindergarten or preschool was however, associated with increased bacterial diversity. Rhinovirus (RV) infection did not have a significant effect on bacterial community composition. A significant difference in bacterial richness was observed between children with RV-A and RV-C infection, however this is likely due to the differences in age group between the patient cohorts. The bacterial community within the oropharynx was found to be diverse and heterogeneous. Age and attendance at day care or kindergarten were important factors in driving bacterial diversity. However, wheeze and viral infection were not found to significantly relate to the bacterial community. Bacterial airway microbiome is highly variable in early life and its role in wheeze remains less clear than viral influences.

Klíčová slova:

Children – Microbiome – Respiratory infections – Ribosomal RNA – Sequence databases – Viral transmission and infection – Bronchiolitis – Rhinovirus infection


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2019 Číslo 10