Armadillo repeat-containing protein 1 is a dual localization protein associated with mitochondrial intermembrane space bridging complex


Autoři: Fabienne Wagner aff001;  Tobias C. Kunz aff001;  Suvagata R. Chowdhury aff001;  Bernd Thiede aff003;  Martin Fraunholz aff001;  Debora Eger aff001;  Vera Kozjak-Pavlovic aff001
Působiště autorů: Department of Microbiology, Biocenter, University of Würzburg, Würzburg, Germany aff001;  German Cancer Research Center (DKFZ), Heidelberg, Germany aff002;  Department of Biosciences, University of Oslo, Oslo, Norway aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0218303

Souhrn

Cristae architecture is important for the function of mitochondria, the organelles that play the central role in many cellular processes. The mitochondrial contact site and cristae organizing system (MICOS) together with the sorting and assembly machinery (SAM) forms the mitochondrial intermembrane space bridging complex (MIB), a large protein complex present in mammalian mitochondria that partakes in the formation and maintenance of cristae. We report here a new subunit of the mammalian MICOS/MIB complex, an armadillo repeat-containing protein 1 (ArmC1). ArmC1 localizes both to cytosol and mitochondria, where it associates with the outer mitochondrial membrane through its carboxy-terminus. ArmC1 interacts with other constituents of the MICOS/MIB complex and its amounts are reduced upon MICOS/MIB complex depletion. Mitochondria lacking ArmC1 do not show defects in cristae structure, respiration or protein content, but appear fragmented and with reduced motility. ArmC1 represents therefore a peripheral MICOS/MIB component that appears to play a role in mitochondrial distribution in the cell.

Klíčová slova:

Cell staining – Confocal microscopy – Cytosol – HeLa cells – Immunoprecipitation – Mitochondria – Outer membrane proteins – Small interfering RNAs


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