Physical exercise stimulates salivary secretion of cystatins

Autoři: Marcelo de Lima Sant’Anna aff001;  Leandro Teixeira Oliveira aff001;  Diego Viana Gomes aff002;  Sergio Tadeu Farinha Marques aff004;  D. William Provance, Jr aff005;  Martha Meriwether Sorenson aff001;  Verônica Pinto Salerno aff002
Působiště autorů: Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil aff001;  Department of Physical Activity Biosciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil aff002;  Almirante Sylvio de Carmargo Training Center, Brazilian Navy, Rio de Janeiro, Brazil aff003;  Naval Sports Commisson, Brazilian Navy, Rio de Janeiro, Brazil aff004;  Center for Technological Development in Health, Oswaldo Cruz Insitute, Rio de Janeiro, Brazil aff005
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224147


Physical exercise is known to activate the sympathetic nervous system, which influences the production of saliva from salivary glands. Our examination of saliva collected from highly trained athletes before and after a number of physical competititions showed an increase in the secretion of S-type cystatins and cystatin C as a subacute response to aerobic and anaerobic exercise. The elevation in salivary cystatins was transient and the recovery time course differed from that of amylase and other salivary proteins. An in vitro assay was developed based on a cell line from a human submandibular gland (HSG) that differentiated into acinus-like structures. Treatments with the β-adrenergic agonist isoproterenol caused a shift in the intracellular distribution of S-type cystatins and cystatin C, promoting their accumulation at the outer regions of the acinus prior to release and suggesting the activation of a directional transport involving co-migration of both molecules. In another treatment using non-differentiated HSG cells, it was evident that both expression and secretion of cystatin C increased upon addition of the β-adrenergic agonist, and these effects were essentially eliminated by the antagonist propranolol. The HSG cell line appears to have potential as a model for exploring the mechanism of cystatin secretion, particularly the S-type cystatins that originate primarily in the submandibular glands.

Klíčová slova:

Exercise – Running – Saliva – Salivary glands – Secretion – Sports – Submandibular glands – Isoproterenol


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2019 Číslo 10