Development of rice conidiation media for Ustilaginoidea virens


Autoři: Yufu Wang aff001;  Fei Wang aff001;  Songlin Xie aff001;  Yi Liu aff001;  Jinsong Qu aff001;  Junbin Huang aff001;  Weixiao Yin aff001;  Chaoxi Luo aff001
Působiště autorů: Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring and Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, China aff001
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0217667

Souhrn

Rice false smut, caused by the ascomycete Ustilaginoidea virens, is a serious disease of rice worldwide. Conidia are very important infectious propagules of U. virens, but the ability of pathogenic isolates to produce conidia frequently decreases in culture, which influences pathogenicity testing. Here, we developed tissue media with rice leaves or panicles that stimulate conidiation of U. virens. Among the tested media, 0.10 g/ml panicle medium was most efficient for conidiation. Whereas, some rice leaf media more effectively increased conidiation than panicle media except 0.10 g/ml panicle medium, and certain non-filtered tissue media were better than their filtered counterparts. Although the conidia induced in rice tissue media were smaller, they were able to germinate on potato sucrose agar medium and infect rice normally. The rice tissue medium is also workable in inducing conidia for conidiation-defective isolates. This method provides a foundation for the production of conidia by U. virens that will be widely applicable in pathogenicity testing as well as in genetic analyses for false smut resistance in rice cultivars.

Klíčová slova:

Fungal sporulation – Leaves – Nutrition – Panicles – Pathogenesis – Potato – Rice – Sucrose


Zdroje

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2019 Číslo 10