Blue-violet light decreases VEGFa production in an in vitro model of AMD

Autoři: Mélanie Marie aff001;  Pauline Gondouin aff001;  Delphine Pagan aff001;  Coralie Barrau aff002;  Thierry Villette aff002;  José Sahel aff001;  Serge Picaud aff001
Působiště autorů: Sorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France aff001;  Essilor International R&D, Charenton-le-Pont, France aff002;  Department of Ophthalmology, The University of Pittsburgh School of Medicine, Pittsburgh, PA, United States of America aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article


Blue light is an identified risk factor for age-related macular degeneration (AMD). The production of vascular endothelial growth factor (VEGF), leading to neovascularization, is a major complication of the wet form of this disease. We investigated how blue light affects VEGF expression and secretion using A2E-loaded retinal pigment epithelium (RPE) cells, a cell model of AMD. Incubation of RPE cells with A2E resulted in a significant increase in VEGF mRNA and, intracellular and secreted VEGF protein levels, but not mRNA levels of VEGFR1 or VEGFR2. Blue light exposure of A2E-loaded RPE cells resulted in a decrease in VEGF mRNA and protein levels, but an increase in VEGFR1 levels. The toxicity of 440 nm light on A2E-loaded RPE cells was enhanced by VEGF supplementation. Our results suggest that age-related A2E accumulation may result in VEGF synthesis and release. This synthesis of VEGF, which enhances blue light toxicity for the RPE cells, is itself suppressed by blue light. Anti-VEGF therapy may therefore improve RPE survival in AMD.

Klíčová slova:

Apoptosis – Light – Macular degeneration – Messenger RNA – Retina – Sunlight – Toxicity – White light


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