Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod Crangonyx islandicus

Autoři: Ragnhildur Guðmundsdóttir aff001;  Agnes-Katharina Kreiling aff001;  Bjarni Kristófer Kristjánsson aff002;  Viggó Þór Marteinsson aff003;  Snæbjörn Pálsson aff001
Působiště autorů: Faculty of Life and Environmental Sciences, University of Iceland, Reykjavík, Iceland aff001;  Department of Aquaculture and Fish Biology, Hólar University, Sauðárkrókur, Iceland aff002;  Matis ohf./Icelandic Food and Biotech R&D, Reykjavík, Iceland aff003;  Faculty of Food Science and Nutrition, University of Iceland, Reykjavík, Iceland aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222527


Crangonyx islandicus is a groundwater amphipod endemic to Iceland, considered to have survived the Ice Ages in subglacial refugia. Currently the species is found in spring sources in lava fields along the tectonic plate boundary of the country. The discovery of a groundwater species in this inaccessible habitat indicates a hidden ecosystem possibly based on chemoautotrophic microorganisms as primary producers. To explore this spring ecosystem, we assessed its microbial diversity and analysed whether and how the diversity varied between the amphipods and the spring water, and if was dependent on environmental factors and geological settings. Isolated DNA from spring water and from amphipods was analysed using metabarcoding methods, targeting the 16S rRNA gene. Two genera of bacteria, Halomonas and Shewanella were dominating in the amphipod samples in terms of relative abundance, but not in the groundwater samples where Flavobacterium, Pseudomonas and Alkanindiges among others were dominating. The richness of the bacteria taxa in the microbial community of the groundwater spring sources was shaped by pH level and the beta diversity was shaped by geographic locations.

Klíčová slova:

Bacteria – Bacterial biofilms – Ecosystems – Spring – Surface water – Iceland – Archaea – Lava


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