Sealing agent reduces formation of single and dual-species biofilms of Candida albicans and Enterococcus faecalis on screw joints at the abutment/implant interface

Autoři: Cecília Alves de Sousa aff001;  Jadison Junio Conforte aff001;  Karina Sampaio Caiaffa aff002;  Cristiane Duque aff002;  Wirley Gonçalves Assunção aff002
Působiště autorů: Department of Dental Materials and Prosthodontic, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil aff001;  Department of Pediatric Dentistry and Public Health, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil aff002
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article


The aim of this research was to evaluate the efficacy of a commercial sealing agent at the abutment/implant interface against microleakage of single and dual-species biofilms of Candida albicans and Enterococcus faecalis into external hexagon (EH) and Morse taper (MT) prosthetic connections. A total of 216 samples of implants and their abutments were tested. Six groups (n = 36) were evaluated based on biofilm and period of incubation (7 and 14 days). The implant connections EH and MT (n = 18) were divided according to the use of the material (n = 9) (EH-T and MT-T: with the sealing agent; EH-C and MT-C: control). The biofilms were analyzed by microbial counting (CFU/mL) and SEM analysis and photographs of the material in the screw joints were also taken. Data were analyzed by Student t test, two-way ANOVA and Bonferroni test. For the single-species biofilms, there was a significant reduction in the growth of E. faecalis when compared MT-C and MT-T or EH-C and EH-T at 7 and 14 days. The same was observed for C. albicans biofilms. For dual-species biofilms of E. faecalis and C. albicans, the sealing agent was more effective in preventing microbial infiltration into the MT connection at 14 days, while microbial infiltration did not occur into EH connections even in absence of the sealing agent for both periods of evaluation. Overall, these data suggest that the presence of the sealing agent reduces or eliminates the microleakage of E. faecalis and C. albicans biofilms into the implants regardless of the period of incubation.

Klíčová slova:

Bacterial biofilms – Biofilms – Candida albicans – Enterococcus faecalis – Medical implants – Prosthetics – Scanning electron microscopy – Bacterial growth


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