Oral delivery of water-soluble compounds to the phytoseiid mite Neoseiulus californicus (Acari: Phytoseiidae)

Autoři: Noureldin A. Ghazy aff001;  Takeshi Suzuki aff001
Působiště autorů: Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan aff001;  Agriculture Zoology Department, Faculty of Agriculture, Mansoura University, El-Mansoura, Egypt aff002;  Japan Society for the Promotion of Science, Chiyoda, Tokyo, Japan aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223929


Phytoseiids are predatory mites that prey on other mites and small arthropods, and several species are used in commercial agriculture for biological control of pests. To optimize phytoseiid mites’ use in biocontrol, an efficient method for oral delivery of test compounds is required to assess their sensitivities to pesticides, RNAi for gene functional analysis and artificial diets. Here we developed four methods for oral delivery of a solution of xenobiotics to different life stages of the commercially available generalist predatory mite Neoseiulus californicus: (i) soaking mites in the solution, or allowing them to feed on (ii) spider mites soaked in the solution, (iii) a solution droplet, or (iv) solution-saturated filter paper. As measured by ingestion of a tracer dye, the droplet-based feeding system was most efficient; the dye was observed in the alimentary canal of >90% test mites of all life stages, with no mortality. The droplet-based feeding system was also effective for the commercially available specialist predatory mite Phytoseiulus persimilis, with >80% delivery efficiency. This study paves the way for development of methods for high-throughput RNAi and for toxicological or nutritional assays in phytoseiid mites.

Klíčová slova:

Filter paper – Larvae – Mites – Pest control – RNA interference – Tracer dye staining – Gene delivery – Liquids


1. van Lenteren JC. The state of commercial augmentative biological control: plenty of natural enemies, but a frustrating lack of uptake. Biocontrol. 2012; 57: 1–20. https://doi.org/10.1007/s10526-011-9395-1

2. McMurtry JA, Croft BA. Life-styles of Phytoseiid mites and their roles in biological control. Annu Rev Entomol. 1997; 42: 291–321. https://doi.org/10.1146/annurev.ento.42.1.291 15012316

3. Rhodes EM, Liburd OE. Evaluation of predatory mites and acramite for control of twospotted spider mites in strawberries in north central Florida. J Econ Entomol. 2006; 99: 1291–1298. https://doi.org/10.1603/0022-0493-99.4.1291 16937684

4. Fraulo AB, Liburd OE. Biological control of twospotted spider mite, Tetranychus urticae, with predatory mite, Neoseiulus californicus, in strawberry. Exp Appl Acarol. 2007; 43: 109–119. https://doi.org/10.1007/s10493-007-9109-7 17924197

5. Gerson U, Weintraub PG. Mites for the control of pests in protected cultivation. Pest Manag Sci. 2007; 63: 658–667. https://doi.org/10.1002/ps.1380 17533640

6. Kola VSR, Renuka P, Madhav MS, Mangrauthia SK. Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing. Front Physiol. 2015; 6: 119. https://doi.org/10.3389/fphys.2015.00119 25954206

7. Yu N, Christiaens O, Liu J, Niu J, Cappelle K, et al. Delivery of dsRNA for RNAi in insects: an overview and future directions. Insect Sci. 2013; 20: 4–14. https://doi.org/10.1111/j.1744-7917.2012.01534.x 23955821

8. Sun D, Guo Z, Liu Y, Zhang Y. Progress and prospects of CRISPR/Cas systems in insects and other arthropods. Front Physiol. 2017; 8: 608. https://doi.org/10.3389/fphys.2017.00608

9. Wu K, Hoy MA. Oral delivery of double-stranded RNA induces prolonged and systemic gene knockdown in Metaseiulus occidentalis only after feeding on Tetranychus urticae. Exp Appl Acarol. 2014; 63: 171–187. https://doi.org/10.1007/s10493-014-9772-4 24509787

10. Ozawa R, Nishimura O, Yazawa S, Muroi A, Takabayashi J, et al. Temperature‐dependent, behavioural, and transcriptional variability of a tritrophic interaction consisting of bean, herbivorous mite, and predator. Mol Ecol. 2012; 21: 5624–5635. https://doi.org/10.1111/mec.12052 23043221

11. McMurtry JA, de Moraes GJ, Sourassou NF. Revision of the lifestyles of phytoseiid mites (Acari: Phytoseiidae) and implications for biological control strategies. Syst Appl Acarol. 2013; 18: 297–320. http://dx.doi.org/10.11158/saa.18.4.1

12. Castagnoli M, Simoni S. Neoseiulus californicus (McGregor) (Acari: Phytoseiidae): survey of biological and behavioral traits of a versatile predator. Redia. 2003; 86: 153–164.

13. Hamlen RA, Lindquist RK. Comparison of two Phytoseiulus species as predators of twospotted spider mites on greenhouse ornamentals. Environ Entomol. 1981; 10: 524–527. https://doi.org/10.1093/ee/10.4.524

14. Sabelis MW. Life history. Capacity for population increase. 1985. In: Helle W, Sabelis MW, editors. Spider mites, their biology, natural enemies and control, vol 1B. Amsterdam: Elsevier. pp. 35–41.

15. Suzuki T, España MU, Nunes MA, Zhurov V, Dermauw W, et al. Protocols for the delivery of small molecules to the two-spotted spider mite, Tetranychus urticae. PLOS ONE. 2017; 12(12): e0190025. https://doi.org/10.1371/journal.pone.0190025 29244858

16. Fife D. Fifer: a biostatisticians toolbox for various activities, including plotting, data cleanup, and data analysis. R package version 1.1. 2017. https://CRAN.R-project.org/package=fifer

17. R Core Team. R: A language and environment for statistical computing. Vienna, Austria; 2017. https://www.R-project.org/

18. Hunter JD. Matplotlib: A 2D Graphics Environment. Comput Sci Eng. 2007; 9: 90–95. https://doi.org/10.1109/MCSE.2007.55

19. Chittenden AR, Saito Y. Why are there feeding and nonfeeding larvae in phytoseiid mites (Acari, Phytoseiidae)? J Ethol. 2001; 19: 55–62. https://doi.org/10.1007/s101640170018

Článek vyšel v časopise


2019 Číslo 10