1. World Health Organization. Guidelines Guideline on When To Start Antiretroviral Therapy and on Pre-Exposure Prophylaxis for Hiv. World Health Organization. 2015; 78. 978 92 4 150956 5
2. Quinn TC, Wawer MJ, Sewankambo N, Serwadda D, Li C, Wabwire-Mangen F, et al. Viral Load and Heterosexual Transmission of Human Immunodeficiency Virus Type 1. New England Journal of Medicine. 2000;342: 921–929. doi: 10.1056/NEJM200003303421303 10738050
3. Monleau M, Aghokeng A. Field evaluation of dried blood spots for routine HIV-1 viral load and drug resistance monitoring in patients receiving antiretroviral therapy in Africa and Asia. Journal of clinical. 2014;
4. Hamers R, Kityo C, Lange J, Wit T de, Mugyenyi P. Global threat from drug resistant HIV in sub-Saharan Africa. Bmj. 2012;
5. Bennett DE, Bertagnolio S, Sutherland D, Gilks CF. The World Health Organization’s global strategy for prevention and assessment of HIV drug resistance Antiretroviral treatment scale-up in resource- limited countries.
6. Bertagnolio S, Parkin NT, Jordan M, Brooks J, Gerardo García-Lerma J. Silvia Bertagnolio, et al.: Dried Blood Spots for HIV-1 Drug Resistance and Viral Load Testing Dried blood spots for HIV-1 Drug Resistance and Viral Load Testing: A Review of Current Knowledge and WHO Efforts for Global HIV Drug Resistance Surveillance. 2010; 21179184
7. WHO. WHO Manual for HIV Drug Resistance Testing Using Dried Blood Spot Specimens. Who/Hiv/201230. 2012; 29.
8. UNAIDS. 90-90-90 An ambitious treatment target to help end the AIDS epidemic. http://wwwunaidsorg/Sites/Default/Files/Media_Asset/90-90-90_En_0Pdf.2014; 40.
9. Ziemniak C, Mengistu Y, Ruff A, Chen Y-H, Khaki L, Bedri A, et al. Use of dried-blood-spot samples and in-house assays to identify antiretroviral drug resistance in HIV-infected children in resource-constrained settings. Journal of clinical microbiology. American Society for Microbiology; 2011;49: 4077–82. doi: 10.1128/JCM.01004-11 21956987
10. Keiser O, Chi BH, Gsponer T, Boulle A, Orrell C, Phiri S, et al. Outcomes of antiretroviral treatment in programmes with and without routine viral load monitoring in Southern Africa. AIDS (London, England). NIH Public Access; 2011;25: 1761–9. doi: 10.1097/QAD.0b013e328349822f 21681057
11. Johannessen A, Garrido C, Zahonero N, Sandvik L, Naman E, Kivuyo SL, et al. Dried Blood Spots Perform Well in Viral Load Monitoring of Patients Who Receive Antiretroviral Treatment in Rural Tanzania. Clinical Infectious Diseases. WHO, Geneva; 2009;49: 976–981. doi: 10.1086/605502 19663598
12. Rottinghaus EEK, Ugbena R, Diallo K, Bassey O, Azeez A, DeVos J, et al. Dried Blood Spot Specimens Are a Suitable Alternative Sample Type for HIV-1 Viral Load Measurement and Drug Resistance Genotyping in Patients Receiving First-Line Antiretroviral Therapy. Clinical Infectious Diseases. 2012;54: 1187–1195. doi: 10.1093/cid/cis015 22412066
13. Sigaloff KCE, Hamers RL, Wallis CL, Kityo C, Siwale M, Ive P, et al. Unnecessary Antiretroviral Treatment Switches and Accumulation of HIV Resistance Mutations; Two Arguments for Viral Load Monitoring in Africa. JAIDS Journal of Acquired Immune Deficiency Syndromes. 2011;58: 23–31. doi: 10.1097/QAI.0b013e318227fc34 21694603
14. World Health Organization. 2010 Revision. Geneva. WHO | Antiretroviral therapy for HIV infection in adults and adolescents. WHO. World Health Organization; 2011;
15. McDade T, Williams S, Snodgrass J. What a drop can do: dried blood spots as a minimally invasive method for integrating biomarkers into population-based research. Demography. 2007;
16. Duncombe C, Rosenblum S, Hellmann N, Holmes C, Wilkinson L, Biot M, et al. Reframing HIV care: putting people at the centre of antiretroviral delivery. Tropical Medicine & International Health. 2015;20: 430–447. doi: 10.1111/tmi.12460 25583302
17. Mavedzenge SN, Davey C, Chirenje T, Mushati P, Mtetwa S, Dirawo J, et al. Finger Prick Dried Blood Spots for HIV Viral Load Measurement in Field Conditions in Zimbabwe. PLoS ONE. Public Library of Science; 2015;10. doi: 10.1371/journal.pone.0126878 26001044
18. Patton JC, Akkers E, Coovadia AH, Meyers TM, Stevens WS, Sherman GG. Evaluation of dried whole blood spots obtained by heel or finger stick as an alternative to venous blood for diagnosis of human immunodeficiency virus type 1 infection in vertically exposed infants in the routine diagnostic laboratory. Clinical and Vaccine Immunology. 2007;14: 201–203. doi: 10.1128/CVI.00223-06 17167036
19. Sherman GGG, Stevens G, Jones SSA, Horsfield P, Stevens WS. Dried blood spots improve access to HIV diagnosis and care for infants in low-resource settings. JAIDS Journal of. 2005;38: 615–617.
20. Holmes CB, Sikazwe I, Sikombe K, Eshun-Wilson I, Czaicki N, Beres LK, et al. Estimated mortality on HIV treatment among active patients and patients lost to follow-up in 4 provinces of Zambia: Findings from a multistage sampling-based survey. Rosen S, editor. PLOS Medicine. Public Library of Science; 2018;15: e1002489. doi: 10.1371/journal.pmed.1002489 29329301
21. Sikazwe I, Eshun-Wilson I, Sikombe K, Czaicki N, Somwe P, Mody A, et al. Retention and viral suppression in a cohort of HIV patients on antiretroviral therapy in Zambia: Regionally representative estimates using a multistage-sampling-based approach. PLOS Medicine. Public Library of Science; 2019;16: 1–17. doi: 10.1371/journal.pmed.1002811 31150380
22. Wolf HT, Halpern-Felsher BL, Bukusi EA, Agot KE, Cohen CR, Auerswald CL. ‘It is all about the fear of being discriminated [against]⋯the person suffering from HIV will not be accepted’: A qualitative study exploring the reasons for loss to follow-up among HIV-positive youth in Kisumu, Kenya. BMC Public Health. BioMed Central; 2014;14: 1154. doi: 10.1186/1471-2458-14-1154 25377362
23. Topp SM, Mwamba C, Sharma A, Mukamba N, Beres LK, Geng E, et al. Rethinking retention: Mapping interactions between multiple factors that influence long-term engagement in HIV care. PLOS ONE. Public Library of Science; 2018;13: e0193641. doi: 10.1371/journal.pone.0193641 29538443
24. Musheke M, Bond V, Merten S. Individual and contextual factors influencing patient attrition from antiretroviral therapy care in an urban community of Lusaka, Zambia. Journal of the International AIDS Society. International AIDS Society; 2012;15. doi: 10.7448/IAS.15.3.17366 22713354
25. Roberts T, Cohn J, Bonner K, Hargreaves S. Scale-up of Routine Viral Load Testing in Resource-Poor Settings: Current and Future Implementation Challenges: Table 1. Clinical Infectious Diseases. Oxford University Press; 2016;62: 1043–1048. doi: 10.1093/cid/ciw001 26743094
26. Rutstein SE, Hosseinipour MC, Kamwendo D, Soko A, Mkandawire M, Biddle AK, et al. Dried Blood Spots for Viral Load Monitoring in Malawi: Feasible and Effective. PLOS ONE. Public Library of Science; 2015;10: e0124748.
27. Morris MB, Chapula BT, Chi BH, Mwango A, Chi HF, Mwanza J, et al. Use of task-shifting to rapidly scale-up HIV treatment services: experiences from Lusaka, Zambia. BMC Health Services Research. 2009;9: 5. doi: 10.1186/1472-6963-9-5 19134202
28. World Health Organization. Technical and operational considerations for implementing HIV viral load testing: Interim technical update. Who. 2014; 28.
29. Hickey MD, Omollo D, Salmen CR, Mattah B, Blat C, Ouma GB, et al. Movement between facilities for HIV care among a mobile population in Kenya: transfer, loss to follow-up, and reengagement. AIDS Care. Taylor & Francis; 2016;28: 1386–1393. doi: 10.1080/09540121.2016.1179253 27145451
30. Geng EH, Odeny TA, Lyamuya R, Nakiwogga-Muwanga A, Diero L, Bwana M, et al. Retention in Care and Patient-Reported Reasons for Undocumented Transfer or Stopping Care Among HIV-Infected Patients on Antiretroviral Therapy in Eastern Africa: Application of a Sampling-Based Approach. Clinical Infectious Diseases. Oxford University Press; 2016;62: 935–944. doi: 10.1093/cid/civ1004 26679625
31. Tweya H, Feldacker C, Estill J, Jahn A, Ng’ambi W, Ben-Smith A, et al. Are They Really Lost? “True” Status and Reasons for Treatment Discontinuation among HIV Infected Patients on Antiretroviral Therapy Considered Lost to Follow Up in Urban Malawi. PLOS ONE. Public Library of Science; 2013;8: e75761. doi: 10.1371/journal.pone.0075761 24086627
32. Grant M, Wilford A, Haskins L, Phakathi S, Mntambo N, Horwood CM. Trust of community health workers influences the acceptance of community-based maternal and child health services. African Journal of Primary Health Care and Family Medicine. 2017; doi: 10.4102/phcfm.v9i1.1281 28582988
33. Sanjana P, Torpey K, Schwarzwalder A, Simumba C, Kasonde P, Nyirenda L, et al. Task-shifting HIV counselling and testing services in Zambia: The role of lay counsellors. Human Resources for Health. 2009; doi: 10.1186/1478-4491-7-44 19480710
34. Kaindjee-Tjituka F, Sawadogo S, Mutandi G, Maher AD, Salomo N, Mbapaha C, et al. Task-shifting point-of-care CD4+ testing to lay health workers in HIV care and treatment services in Namibia. African Journal of Laboratory Medicine. 2017; doi: 10.4102/ajlm.v6i1.643 29159139
35. Working Group on Modelling of Antiretroviral Therapy Monitoring Strategies in Sub-Saharan Africa A, Phillips A, Shroufi A, Vojnov L, Cohn J, Roberts T, et al. Sustainable HIV treatment in Africa through viral-load-informed differentiated care. Nature. NIH Public Access; 2015;528: S68–S76. doi: 10.1038/nature16046 26633768
36. UNAIDS. How Aids Changed Everything [Internet]. 2015. Available: http://www.unaids.org/sites/default/files/media_asset/MDG6Report_en.pdf
37. Raizes E, Hader S, Birx D. Expansion of Viral Load Testing and the Potential Impact on HIV Drug Resistance. The Journal of infectious diseases. Oxford University Press; 2017;216: S805–S807. doi: 10.1093/infdis/jix432 29206999
38. Rachlis B, Ochieng D, Geng E, Rotich E, Ochieng V, Maritim B, et al. Implementation and operational research: evaluating outcomes of patients lost to follow-up in a large comprehensive care treatment program in western Kenya. Journal of acquired immune deficiency syndromes (1999). 2015;68: e46–55. doi: 10.1097/QAI.0000000000000492 25692336
39. Chimbindi N, Bärnighausen T, Newell M-L. Patient satisfaction with HIV and TB treatment in a public programme in rural KwaZulu-Natal: evidence from patient-exit interviews. BMC Health Services Research. 2014;14: 32. doi: 10.1186/1472-6963-14-32 24450409
40. Dang BN, Westbrook RA, Black WC, Rodriguez-Barradas MC, Giordano TP. Examining the Link between Patient Satisfaction and Adherence to HIV Care: A Structural Equation Model. PLoS ONE. 2013;8. doi: 10.1371/journal.pone.0054729 23382948
41. Mody A, Sikazwe I, Czaicki NL, Wa Mwanza M, Savory T, Sikombe K, et al. Estimating the real-world effects of expanding antiretroviral treatment eligibility: Evidence from a regression discontinuity analysis in Zambia. PLoS Medicine. 2018;15. doi: 10.1371/journal.pmed.1002574 29870531
42. Boahen O, Owusu-Agyei S, Febir LG, Tawiah C, Tawiah T, Afari S, et al. Community perception and beliefs about blood draw for clinical research in Ghana. Transactions of the Royal Society of Tropical Medicine and Hygiene. Oxford University Press; 2013;107: 261–265. doi: 10.1093/trstmh/trt012 23426114
43. Berger BE, Ferrans CE, Lashley FR. Measuring stigma in people with HIV: Psychometric assessment of the HIV stigma scale. Research in Nursing & Health. Wiley Online Library; 2001;24: 518–529. doi: 10.1002/nur.10011 11746080
44. Dorward J, Drain PK, Garrett N. Point-of-care viral load testing and differentiated HIV care. The Lancet HIV. 2018. pp. e8–e9. doi: 10.1016/S2352-3018(17)30211-4 29290227