Nutrigenomic effect of conjugated linoleic acid on growth and meat quality indices of growing rabbit


Autoři: A. M. Abdelatty aff001;  Shereen A. Mohamed aff002;  Mahmoud M. A. Moustafa aff002;  Asmaa K. Al-Mokaddem aff003;  M. R. Baker aff004;  Ahmed A. Elolimy aff005;  Shawky A. Elmedany aff006;  Shaymaa Hussein aff007;  Omar A. A. Farid aff008;  Osama G. Sakr aff009;  Mohamed A. Elhady aff010;  Massimo Bionaz aff011
Působiště autorů: Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff001;  Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Qalyubia, Egypt aff002;  Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza,Egypt aff003;  Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff004;  Department of Animal Sciences, University of Illinois, Urbana, IL, United States of America aff005;  Regional Center for Food and Feed, Agriculture Research Center; Giza, Egypt aff006;  Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff007;  Department of Physiology, National Organization for Drug Control and Research, Giza, Egypt aff008;  Animal Production Department (Poultry Physiology), Faculty of Agriculture, Cairo University, Giza, Egypt aff009;  Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff010;  Department of Animal and Rangeland sciences, Oregon State University, Oregon, United States of America aff011
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222404

Souhrn

Conjugated linoleic acid was detected in rabbit caecotrophs, due to the presence of microbial lipid activity in rabbit cecum. However, the effect of CLA as a functional food in growing rabbit is not well established. Therefore, this study was conducted to determine the effect of CLA on production, meat quality, and its nutrigenomic effect on edible parts of rabbit carcass including skeletal muscle, liver, and adipose tissue. Therefore, seventy five weaned V-Line male rabbits, 30 days old, were randomly allocated into three dietary treatments receiving either basal control diet, diet supplemented with 0.5% (CLAL), or 1% CLA (CLAH). Total experimental period (63 d) was segmented into 7 days adaptation and 56 days experimental period. Dietary supplementation of CLA did not alter growth performance, however, the fat percentage of longissimus lumborum muscle was decreased, with an increase in protein and polyunsaturated fatty acids (PUFA) percentage. Saturated fatty acids (SFA) and mono unsaturated fatty acids (MUFA) were not increased in CLA treated groups. There was tissue specific sensing of CLA, since subcutaneous adipose tissue gene expression of PPARA was downregulated, however, CPT1A tended to be upregulated in liver of CLAL group only (P = 0.09). In skeletal muscle, FASN and PPARG were upregulated in CLAH group only (P ≤0.01). Marked cytoplasmic vacuolation was noticed in liver of CLAH group without altering hepatocyte structure. Adipocyte size was decreased in CLA fed groups, in a dose dependent manner (P <0.01). Cell proliferation determined by PCNA was lower (P <0.01) in adipose tissue of CLA groups. Our data indicate that dietary supplementation of CLA (c9,t11-CLA and t10,c12- CLA) at a dose of 0.5% in growing rabbit diet produce rabbit meat rich in PUFA and lower fat % without altering growth performance and hepatocyte structure.

Klíčová slova:

Adipose tissue – Diet – Fats – Fatty acids – Meat – Rabbits – Skeletal muscles – Oleic acid


Zdroje

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