Localization and characterization of thyroid microcalcifications: A histopathological study

Autoři: Joanne Guerlain aff001;  Sophie Perie aff002;  Marine Lefevre aff003;  Joëlle Perez aff001;  Sophie Vandermeersch aff001;  Chantal Jouanneau aff003;  Léa Huguet aff001;  Vincent Frochot aff004;  Emmanuel Letavernier aff001;  Raphael Weil aff005;  Stéphan Rouziere aff005;  Dominique Bazin aff006;  Michel Daudon aff004;  Jean-Philippe Haymann aff001
Působiště autorů: Sorbonne Université, INSERM, UMR_S 1155, AP-HP, Hôpital Tenon, Paris, France aff001;  Service d’Oto-rhino-laryngologie et de Chirurgie Cervico-Faciale, Hôpital Tenon, Paris, France aff002;  Service d’Anatomopathologie, Hôpital Tenon, Paris, France aff003;  Service d’Explorations Fonctionnelles Multidisciplinaires, Assistance Publique—Hôpitaux de Paris (AP-HP), Hôpital Tenon, Paris, France aff004;  Laboratoire de Physique des Solides, UMR CNRS 8502, Université Paris Sud, Université Paris Saclay, Orsay Cedex, France aff005;  Laboratoire de Chimie Physique, Université Paris Sud, Orsay, France aff006
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224138


Thyroid calcification is frequent in thyroid nodules. The aim of our study was to evaluate the prevalence of calcifications in thyroid tissue samples of patients with various thyroid diseases, and to identify their composition according to their localization. Among 50 thyroid samples included, 56% were malignant (papillary carcinoma) and 44% were benign (adenoma, multinodular goiter, Graves’ disease, sarcoidosis). Calcifications were found in 95% of samples using polarised light microscopy, whereas only 12% were described in initial pathological reports. Three types were individualised and analyzed by infrared spectrometry (μFTIR): colloid calcifications composed of calcium oxalate, capsular calcifications and psammoma bodies, both composed of calcium phosphate. Of notice, psammoma bodies characterized by FE-SEM were composed of concentric structure suggesting a slow process for crystal deposition. Calcium phosphates were found only in malignant samples whereas calcium oxalate was not associated with a define pathology. Proliferation assessed by KI67 staining was high (33% of positive follicles), and RUNX2, OPN, and CD44 positive staining were detected in thyrocytes with a broad variation between samples. However, thyrocyte proliferation and differentiation markers were not associated with the number of crystals. TRPV5 and CaSR expression was also detected in thyrocytes. mRNA transcripts expression was confirmed in a subgroup of 10 patients, altogether with other calcium transporters such as PMCA1 or Cav1.3. Interestingly, TRPV5 mRNA expression was significantly associated with number of colloid calcifications (rho = -0.72; p = 0.02). The high prevalence of calcium oxalate crystals within colloid gel raises intriguing issues upon follicle physiology for calcium and oxalate transport.

Klíčová slova:

Calcification – Colloids – Crystal structure – Crystals – Oxalates – Thyroid – Polarized light microscopy – Sense strands


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2019 Číslo 10