Tissue-specific expression of IgG receptors by human macrophages ex vivo

Autoři: Christine W. Bruggeman aff001;  Julia Houtzager aff002;  Barbara Dierdorp aff003;  Jesper Kers aff004;  Steven T. Pals aff004;  René Lutter aff003;  Thomas van Gulik aff002;  Joke M. M. den Haan aff006;  Timo K. van den Berg aff001;  Robin van Bruggen aff001;  Taco W. Kuijpers aff001
Působiště autorů: Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands aff001;  Department of Experimental Surgery, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands aff002;  Department of Experimental Immunology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands aff003;  Department of Pathology, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands aff004;  Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, Amsterdam, The Netherlands aff005;  Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands aff006;  Emma Children's Hospital, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands aff007
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223264


Recently it was discovered that tissue-resident macrophages derive from embryonic precursors, not only from peripheral blood monocytes, and maintain themselves by self-renewal. Most in-vitro studies on macrophage biology make use of in-vitro cultured human monocyte-derived macrophages. Phagocytosis of IgG-opsonized particles by tissue-resident macrophages takes place via interaction with IgG receptors, the Fc-gamma receptors (FcγRs). We investigated the FcγR expression on macrophages both in-vivo and ex-vivo from different human tissues. Upon isolation of primary human macrophages from bone marrow, spleen, liver and lung, we observed that macrophages from all studied tissues expressed high levels of FcγRIII, which was in direct contrast with the low expression on blood monocyte-derived macrophages. Expression levels of FcγRI were highly variable, with bone marrow macrophages showing the lowest and alveolar macrophages the highest expression. Kupffer cells in the liver were the only tissue-resident macrophages that expressed the inhibitory IgG receptor, FcγRIIB. This inhibitory receptor was also found to be expressed by sinusoidal endothelial cells in the liver. In sum, our immunofluorescence data combined with ex-vivo stainings of isolated macrophages indicated that tissue-resident macrophages are remarkably unique and different from monocyte-derived macrophages in their phenotypic expression of IgG receptors. Tissue macrophages show distinct tissue-specific FcγR expression patterns.

Klíčová slova:

Alveolar macrophages – Blood – Cloning – Flow cytometry – Macrophages – Monocytes – Spleen – Kupffer cells


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2019 Číslo 10