Structural characterization of a novel polysaccharide from Sargassum thunbergii and its antioxidant and anti-inflammation effects

Autoři: Dianhui Luo aff001;  Zhaojing Wang aff001;  Kaiying Nie aff001
Působiště autorů: Department of Bioengineering and Biotechnology, Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian, People’s Republic of China aff001
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223198


A novel polysaccharide STSP-I was isolated and purified from Sargassum thunbergii. Its structure and bioactivity were studied using gas chromatography (GC), fourier transform infrared spectroscopy (FTIR), periodate oxidation-smith degradation, partial acid hydrolysis, methylation-GC-MS, nuclear magnetic resonance (NMR), transmission electron microscopy (TEM), radicals scavenging assays and anti-inflammatory assays. STSP-I was consisted of fucose and galactose with a molar ratio of 1.2:1, and its mass was 373 kDa. The main structural components of STSP-I were →4)-α-D-Galp-(1→ and →3)-β-L-Fucp-(1→, STSP-I was a non-branched polysaccharide, and TEM further revealed the existence of entangled chains and linear forms. Compared with Vitamin C (Vc), STSP-I showed a higher scavenging effect of superoxide radical (EC50 = 0.22 mg/mL) and an equivalent scavenging effect of hydroxyl radical (EC50 = 0.88 mg/mL). STSP-I also exhibited good inhibitory effects of TNF-α, IL-6 and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 mouse macrophage cells, and the inhibitory effects were more than 91% at the concentrations of 75 and 150 μg/ml. The results indicate that the polysaccharide STSP-I from S. thunbergii with the linear structure may serve as potential antioxidant and anti-inflammatory agents.

Klíčová slova:

Antioxidants – Macrophages – Methylation – Polysaccharides – Galactose – Hydroxyl radicals – Total correlation spectroscopy – Hydrolysis


1. Khan SB, Kong CS, Kim JA, Kim SK. Protective effect of Amphiroa dilatata on ROS-induced oxidative damage and MMP expressions in HT1080 cells. Biotechnol. Bioprocess Eng. 2010; 15: 191–198.

2. Tang ZH, Liu ZY, Wang J, Zhang N, Mao YF, Guo L, et al. Development of Sargassum thunbergii Microsatellites and Genetic Diversity Description of Qingdao Population. Periodical Ocean Univ. China 2015; 45 (1): 35–40.

3. Wei YX, Li J, Wang JC, Qi HT. Screening of different fractions of phlorotannins from Sargassum thunbergii kuntze for anticoagulant activity. Chin. J. Biochem. Pharm. 2007; 28: 227–229.

4. Jiang Q, Liu D, Yang JB, Yan PC, Huang KX, Lin WH. Chemical constituents from marine alga Sargassum thunbergii. Chin. Pharm. J. 2012; 47: 948–952.

5. Yang FM, Wang L, Hu QH. Preparation of Polysaccharide Derived from Sargassum thunbergii and Its Antioxidant Activity. Food Sci. 2005; 26 (2): 224–227.

6. Huang ZX, Mei XT, Xu DH, Xu SB, Lv JY. Protective effects of polysacchride of Spirulina platensis and Sargassum thunbeergii on vascular of alloxan induced diabetic rats. China. J. Chin. Mater. Med. 2005; 30: 211–215.

7. Wei YX, Li J, Zhao AY, Wang CY, Yu SG, Cong PJ. Preliminary studies on the preparation of polysaccharide from Kuntze (PST) and its anticoagulation activity. Chin. J. Mar. Drugs 2006; 25: 41–44.

8. Fu X, Cao CL, Ren BB, Zhang B, Huang Q, Li C. Structural characterization and in vitro fermentation of a novel polysaccharide from Sargassum thunbergii and its impact on gut microbiota. Carbohydr. Polym. 2018; 183: 230–239. doi: 10.1016/j.carbpol.2017.12.048 29352879

9. Jin WH, Liu B, Li S, Chen J, Tang H, Jiang D, et al. The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities. Int. J. Biol. Macromol. 2018; 108: 307–313. doi: 10.1016/j.ijbiomac.2017.12.009 29217183

10. Jin WH, Wu WL, Tang H, Wei B, Wang H, Sun JD, et al. Structure analysis and anti-tumor and anti-angiogenic activities of sulfated galactofucan extracted from Sargassum thunbergii. Mar. Drugs 2019; 17: 52.

11. Zhuang C, Itoh H, Mizuno T, Ito H. Antitumor active fucoidan from the brown seaweed, Umitoranoo (Sargassum thunbergii). Biosci. Biotech. Biochem. 1995; 59 (4): 563–567.

12. Yuan X.M, Zeng YW, Nie KY, Luo DH, Wang ZJ. Extraction optimization, characterization and bioactivities of a major polysaccharide from Sargassum thunbergii. PLoS One 2015; 10 (12): e0144773. doi: 10.1371/journal.pone.0144773 26649576

13. Luo DH, Yuan XM, Zeng YW, Nie KY, Li ZM, Wang ZJ. Structure elucidation of a major fucopyranose-rich heteropolysaccharide (STP-II) from Sargassum thunbergii. Carbohydr. Polym. 2016; 143: 1–8. doi: 10.1016/j.carbpol.2016.01.049 27083337

14. Dung NT, Bajpai VK, Yoon JI, Kang SC. Anti-inflammatory effects of essential oil isolated from the buds of Cleistocalyx operculatus (Roxb.) Merr and Perry. Food Chem. Toxicol. 2009; 47: 449–453. doi: 10.1016/j.fct.2008.11.033 19095037

15. Lee MY, Lee JA, Seo CS, Ha H, Lee H, Son JK, et al. Antiinflammatory activity of Angelica dahurica ethanolic extract on RAW 264.7 cells via upregulation of heme oxygenase-1. Food Chem. Toxicol. 2011; 49: 1047–1055. doi: 10.1016/j.fct.2011.01.010

16. Poltorak A, He XL, Smirnova I, Liu MY, Van Huffel C, Du X, et al. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: Mutations in Tlr4 gene. Sci. 1998; 282: 2085–2088.

17. Kanno SI, Shouji A, Tomizawa A, Hiura T, Osanai Y, Ujibe M, et al. Inhibitory effect of naringin on lipopolysaccharide (LPS)-induced endotoxin shock in mice and nitric oxide production in RAW 264.7 macrophages. Life Sci. 2006; 78: 673–681. doi: 10.1016/j.lfs.2005.04.051 16137700

18. Coker RK, Laurent GJ. Pulmonary fibrosis: cytokines in the balance. Eur. Resp. J. 11(6) (1998) 1218–1221.

19. Kern TS. Contributions of inflammatory processes to the development of the early stages of diabetic retinopathy. Exp. Diabetes. Res. 2007; 1: 95103–95116.

20. Schroder K, Sweet MJ, Hume DA. Signal integration between IFNc and TLR signaling pathways in macrophages. Immunobiology 2006; 211: 511–524. doi: 10.1016/j.imbio.2006.05.007

21. Kazłowska K, Hsu T, Hou CC, Yang WC, Tsai GJ. Anti-inflammatory properties of phenolic compounds and crude extract from Porphyradentata. J. Ethnopharmacol. 2010; 128: 123–130. doi: 10.1016/j.jep.2009.12.037 20051261

22. Mueller M, Hobiger S, Jungbauer A. Anti-inflammatory activity of extracts from fruits, herbs and spices. Food Chem. 2010; 122: 987–996.

23. Van Q, Nayak BN, Reimer M, Jones PJH, Fulcher RG, Rempel CB. Anti-inflammatory effect of Inonotus obliquus, Polygala senega L., and Viburnum trilobum in a cell screening assay. J. Ethnopharmacol. 2009; 125: 487–493. doi: 10.1016/j.jep.2009.06.026 19577624

24. Luo DH, Fang BS. Structural identification of ginseng polysaccharides and testing of their antioxidant activities. Carbohydr. Polym. 2008; 72: 376–381.

25. Wang ZY, Zhou F, Quan Y. Antioxidant and immunological activity in vitro of polysaccharides from Phellinus nigricans mycelia. Int. J. Biol. Macromol. 2014; 64: 139–143. doi: 10.1016/j.ijbiomac.2013.11.038 24321489

26. Dubois M, Gillis KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956; 28: 350–356.

27. Bradford MM. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976; 72: 248–254. doi: 10.1006/abio.1976.9999 942051

28. Wang L, Li XX, Chen ZX. Sulfated modification of the polysaccharides obtained from defatted rice bran and their antitumor activities. Int. J. Biol. Macromol. 2009; 44: 211–214. doi: 10.1016/j.ijbiomac.2008.12.006 19135473

29. Wang ZJ, Luo DH. Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino. Carbohydr. Polym. 2007; 68: 54–58.

30. Luo DH, Wang ZJ, Li ZM, Yu XQ. Structure of an entangled heteropolysaccharide from Pholidota chinensis Lindl and its antioxidant and anti-cancer properties. Int. J. Biol. Macromol. 2018; 112: 921–928. doi: 10.1016/j.ijbiomac.2018.02.051 29432833

31. Liu J, Wen XY, Kan J, Jin CH. Structural characterization of two water-soluble polysaccharides from black soybean (Glycine max (L.) Merr.). J. Agric. Food Chem. 2015; 63: 225–234. doi: 10.1021/jf505172m 25494923

32. Pramanik M, Mondal S, Chakraborty I, Rout D, Islam SS. Structural investigation of polysaccharide (Fr. II) isolation from the aqueous extract of an edible mushroom, Pleurotus sajor-caju. Carbohydr. Res. 2005; 340: 629–636. doi: 10.1016/j.carres.2004.12.032

33. Li B, Wei XJ, Sun JL, Xu SY. Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed, Hizikia fusiforme. Carbohydr. Res. 2006; 341: 1135–1146. doi: 10.1016/j.carres.2006.03.035 16643873

34. Chen L, Liu J, Zhang Y, Dai B, An Y, Yu L. Structural, thermal, and anti-inflammatory properties of a novel pectic polysaccharide from alfalfa (Medicago sativa L.) stem. J. Agric. Food Chem. 2015; 63: 3219–3228. doi: 10.1021/acs.jafc.5b00494 25756601

35. Candiracci M, Piatti E, Dominguez-Barragaán M, García-Antraás D, Morgado B, Ruano D, et al. Anti-inflammatory Activity of a Honey Flavonoid Extract on Lipopolysaccharide-Activated N13 Microglial Cells. J. Agric. Food Chem. 2012; 60: 12304–12311. doi: 10.1021/jf302468h 23176387

36. Yang F, Shi HM, Zhang XW, Yu LL. Two Novel Anti-Inflammatory 21-Nordammarane Saponins from Tetraploid Jiaogulan (Gynostemma pentaphyllum). J. Agric. Food Chem. 2013; 61: 12646–12652. doi: 10.1021/jf404726z 24320209

37. Zhang XW, Gao BY, Shi HM, Slavin M, Huang HQ, Whent M, et al. Chemical Composition of 13 Commercial Soybean Samples and Their Antioxidant and Anti-inflammatory Properties. J. Agric. Food Chem. 2012; 60: 10027–10034. doi: 10.1021/jf303039a 22978480

38. Jing YS, Zhu JH, Liu T, Bi SX, Hu XJ, Chen ZY, et al. Structural Characterization and Biological Activities of a Novel Polysaccharide from Cultured Cordyceps militaris and Its Sulfated Derivative. J. Agric. Food Chem. 2015; 63: 3464–3471. doi: 10.1021/jf505915t 25785351

39. Kocharova NA, Ovchinnikova OG, Bushmarinov IS, Toukach FV, Torzewska A, Shashkov AS, et al. The structure of the O-polysaccharide from the lipopolysaccharide of Providencia stuartii O57 containing an amide of D-galacturonic acid with L-alanine. Carbohydr. Res. 2005; 340: 775–780. doi: 10.1016/j.carres.2005.01.007 15721353

40. Chen L, Cheung PCK. Mushroom dietary fiber from the fruiting body of pleurotus tuber-regium: fractionation and structural elucidation of nondigestible cell wall components. J. Agric. Food Chem. 2014; 62: 2891–2899. doi: 10.1021/jf500112j 24625260

41. Wang K, Li W, Rui X, Li T, Chen X, Jiang M, et al. Chemical modification, characterization and bioactivity of a released exopolysaccharide (r-EPS1) from Lactobacillus plantarum 70810. Glycoconj. J. 2015; 32: 17–27. doi: 10.1007/s10719-014-9567-1 25417068

Článek vyšel v časopise


2019 Číslo 10

Nejčtenější v tomto čísle

Tomuto tématu se dále věnují…


Zvyšte si kvalifikaci online z pohodlí domova

Léčba bolesti v ordinaci praktického lékaře
nový kurz
Autoři: MUDr. PhDr. Zdeňka Nováková, Ph.D.

Revmatoidní artritida: včas a k cíli
Autoři: MUDr. Heřman Mann

Jistoty a nástrahy antikoagulační léčby aneb kardiolog - neurolog - farmakolog - nefrolog - právník diskutují
Autoři: doc. MUDr. Štěpán Havránek, Ph.D., prof. MUDr. Roman Herzig, Ph.D., doc. MUDr. Karel Urbánek, Ph.D., prim. MUDr. Jan Vachek, MUDr. et Mgr. Jolana Těšínová, Ph.D.

Léčba akutní pooperační bolesti
Autoři: doc. MUDr. Jiří Málek, CSc.

Nové antipsychotikum kariprazin v léčbě schizofrenie
Autoři: prof. MUDr. Cyril Höschl, DrSc., FRCPsych.

Všechny kurzy
Kurzy Doporučená témata Časopisy
Zapomenuté heslo

Nemáte účet?  Registrujte se

Zapomenuté heslo

Zadejte e-mailovou adresu se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.


Nemáte účet?  Registrujte se