The effects of environmental parameters on the microbial activity in peat-bog lakes

Autoři: Sylwia Lew aff001;  Katarzyna Glińska-Lewczuk aff002;  Marcin Lew aff003
Působiště autorů: University of Warmia and Mazury in Olsztyn, Department of Microbiology and Mycology, Faculty of Biology and Biotechnology, Olsztyn, Poland aff001;  University of Warmia and Mazury in Olsztyn, Department of Water Resources, Climatology and Environmental Management, Faculty of Environmental Management and Agriculture, Olsztyn, Poland aff002;  University of Warmia and Mazury in Poland, Faculty of Veterinary Medicine, Olsztyn, Poland aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224441


Microbiological activity is an important parameter for understanding the functioning of different environments. Therefore, the purpose of this study was to estimate the quantity and contribution of metabolically active at the single-cell level bacteria in the microbial community in peat-bog lakes. To determine different aspects of the metabolic activity of bacteria, four fluorescent staining methods (Dehydrogenase/Electron Transport System Activity -CTC+, Nucleoid Containing Cells- NuCC+, Active Bacteria with Intact Ribosome Structures- RIB+ and Active Bacteria With an Intact Membrane—MEM+) were applied. We identified four natural peat-bog lakes in Northern Europe to determine which factors—community (bacterial factors) or environment (hydrochemical and physical factors)—have a significant influence on the quantitative dynamics of metabolically active microorganisms, in terms of seasonal and habitat changes. The results show that change in the amount of abiotic components such as DOC, TN, and TOC can result in stress, which may limit a function but does not lead to losing all other metabolic functions in the community-forming bacteria. In nutrient-poor peat bog lakes, nutrients and organic carbon are factors which regulate the overall activity of the community.

Klíčová slova:

Bacteria – Cell membranes – Cell metabolism – Cell staining – Lakes – Surface water – Bogs – Bacterioplankton


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