Positive effect of an electrolyzed reduced water on gut permeability, fecal microbiota and liver in an animal model of Parkinson’s disease


Autoři: Laura Bordoni aff001;  Rosita Gabbianelli aff001;  Donatella Fedeli aff001;  Dennis Fiorini aff002;  Ina Bergheim aff003;  Cheng Jun Jin aff004;  Lisa Marinelli aff005;  Antonio Di Stefano aff005;  Cinzia Nasuti aff006
Působiště autorů: School of Pharmacy, Molecular Biology Unit, University of Camerino, Camerino, Italy aff001;  School of Science and Technology, Chemistry Unit, University of Camerino, Camerino, Italy aff002;  Department of Nutritional Sciences, RF Molecular Nutritional Science, University of Vienna, Vienna, Austria aff003;  Institute of Nutritional Sciences, SD Model Systems of Molecular Nutrition, Friedrich-Schiller-University, Jena, Germany aff004;  Department of Pharmacy, University of "G. D’Annunzio", Chieti, Italy aff005;  School of Pharmacy, Pharmacology Unit, University of Camerino, Camerino, Italy aff006
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223238

Souhrn

There is growing awareness within the scientific community of the strong connection between the inflammation in the intestine and the pathogenesis of Parkinson’s disease (PD). In previous studies we developed a PD animal model exposing pup rats to permethrin (PERM) pesticide. Here, we intended to explore whether in our animal model there were changes in gut permeability, fecal microbiota and hepatic injury. Moreover, we tested if the co-treatment with an electrolyzed reduced (ERW) was effective to protect against alterations induced by PERM. Rats (from postnatal day 6 to 21) were gavaged daily with PERM, PERM+ERW or vehicle and gut, liver and feces were analyzed in 2-months-old rats. Increased gut permeability, measured by FITC-dextran assay, was detected in PERM group compared to control and PERM+ERW groups. In duodenum and ileum, concentration of occludin was higher in control group than those measured in PERM group, whereas only in duodenum ZO-1 was higher in control than those measured in PERM and PERM+ERW groups. Number of inflammatory focis and neutrophils as well as iNOS protein levels were higher in livers of PERM-treated rats than in those of PERM+ERW and control rats. Fecal microbiota analysis revealed that Lachnospira was less abundant and Defluviitaleaceae more abundant in the PERM group, whereas the co-treatment with ERW was protective against PERM treatment since the abundances in Lachnospira and Defluviitaleaceae were similar to those in the control group. Higher abundances of butyrate- producing bacteria such as Blautia, U.m. of Lachnospiraceae family, U.m. of Ruminococcaceae family, Papillibacter, Roseburia, Intestinimonas, Shuttleworthia together with higher butyric acid levels were detected in PERM+ERW group compared to the other groups. In conclusion, the PD animal model showed increased intestinal permeability together with hepatic inflammation correlated with altered gut microbiota. The positive effects of ERW co-treatment observed in gut, liver and brain of rats were linked to changes on gut microbiota.

Klíčová slova:

Animal models – Bacteria – Duodenum – Gastrointestinal tract – Ileum – Microbiome – Parkinson disease – Permeability


Zdroje

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PLOS One


2019 Číslo 10

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