Genetic diversification of Panstrongylus geniculatus (Reduviidae: Triatominae) in northern South America


Autoři: Valentina Caicedo-Garzón aff001;  Fabian C. Salgado-Roa aff002;  Melissa Sánchez-Herrera aff002;  Carolina Hernández aff001;  Luisa María Arias-Giraldo aff001;  Lineth García aff003;  Gustavo Vallejo aff004;  Omar Cantillo aff005;  Catalina Tovar aff006;  Joao Aristeu da Rosa aff007;  Hernán J. Carrasco aff008;  Maikell Segovia aff008;  Camilo Salazar aff002;  Juan David Ramírez aff001
Působiště autorů: Grupo de Investigaciones Microbiológicas–UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Cra. Bogotá D.C., Colombia aff001;  Grupo de Genética Evolutiva, Filogeografía y Ecología de la Biodiversidad Neotropical (GEUR), Departamento de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Bogotá D.C., Colombia aff002;  Universidad Nacional de San Simón, Cochabamba, Bolivia aff003;  Laboratorio de Investigaciones en Parasitología Tropical (LIPT), Universidad del Tolima, Ibagué, Colombia aff004;  Laboratorio de Referencia e Investigación en Enfermedades Tropicales, Dirección de Sanidad Ejército, Ejército Nacional de Colombia, Bogotá, Colombia aff005;  Grupo de investigación en Enfermedades Tropicales y Resistencia Bacteriana, Programa de Medicina, Facultad de Ciencias de la Salud, Universidad del Sinú, Montería, Colombia aff006;  Laboratório de Parasitologia, Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Araraquara, SP, Brasil aff007;  Laboratorio de Biología Molecular de Protozoarios, Instituto de Medicina Tropical, Universidad Central de Venezuela, Caracas, Venezuela aff008
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223963

Souhrn

Triatomines are the vectors of Trypanosoma cruzi, the etiological agent of Chagas disease. Although Triatoma and Rhodnius are the most-studied vector genera, other triatomines, such as Panstrongylus, also transmit T. cruzi, creating new epidemiological scenarios. Panstrongylus has at least 13 reported species but there is limited information about its intraspecific genetic variation and patterns of diversification. Here, we begin to fill this gap by studying populations of P. geniculatus from Colombia and Venezuela and including other epidemiologically important species from the region. We examined the pattern of diversification of P. geniculatus in Colombia using mitochondrial and nuclear ribosomal data. Genetic diversity and differentiation were calculated within and among populations of P. geniculatus. Moreover, we constructed maximum likelihood and Bayesian inference phylogenies and haplotype networks using P. geniculatus and other species from the genus (P. megistus, P. lignarius, P. lutzi, P. tupynambai, P. chinai, P. rufotuberculatus and P. howardi). Using a coalescence framework, we also dated the P. geniculatus lineages. The total evidence tree showed that P. geniculatus is a monophyletic species, with four clades that are concordant with its geographic distribution and are partly explained by the Andes orogeny. However, other factors, including anthropogenic and eco-epidemiological effects must be investigated to explain the existence of recent geographic P. geniculatus lineages. The epidemiological dynamics in structured vector populations, such as those found here, warrant further investigation. Extending our knowledge of P. geniculatus is necessary for the accurate development of effective strategies for the control of Chagas disease vectors.

Klíčová slova:

Genetic loci – Haplotypes – Mitochondria – Paleogenetics – Phylogenetic analysis – Phylogeography – Population genetics – Ribosomal RNA


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