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APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy


Autoři: Leslie A. Bruggeman aff001;  Zhenzhen Wu aff001;  Liping Luo aff001;  Sethu Madhavan aff003;  Paul E. Drawz aff004;  David B. Thomas aff005;  Laura Barisoni aff006;  John F. O'Toole aff001;  John R. Sedor aff001
Působiště autorů: Departments of Inflammation & Immunity and Nephrology, Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America aff001;  Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America aff002;  Department of Medicine, Ohio State University, Columbus, Ohio, United States of America aff003;  Department of Medicine, University of Minnesota, Minneapolis, Minnesota, United States of America aff004;  Departments of Pathology, University of Miami, Miami, Florida, United States of America aff005;  Departments of Pathology and Medicine, Duke University, Durham, North Carolina, United States of America aff006
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0224408

Souhrn

African polymorphisms in the gene for Apolipoprotein L1 (APOL1) confer a survival advantage against lethal trypanosomiasis but also an increased risk for several chronic kidney diseases (CKD) including HIV-associated nephropathy (HIVAN). APOL1 is expressed in renal cells, however, the pathogenic events that lead to renal cell damage and kidney disease are not fully understood. The podocyte function of APOL1-G0 versus APOL1-G2 in the setting of a known disease stressor was assessed using transgenic mouse models. Transgene expression, survival, renal pathology and function, and podocyte density were assessed in an intercross of a mouse model of HIVAN (Tg26) with two mouse models that express either APOL1-G0 or APOL1-G2 in podocytes. Mice that expressed HIV genes developed heavy proteinuria and glomerulosclerosis, and had significant losses in podocyte numbers and reductions in podocyte densities. Mice that co-expressed APOL1-G0 and HIV had preserved podocyte numbers and densities, with fewer morphologic manifestations typical of HIVAN pathology. Podocyte losses and pathology in mice co-expressing APOL1-G2 and HIV were not significantly different from mice expressing only HIV. Podocyte hypertrophy, a known compensatory event to stress, was increased in the mice co-expressing HIV and APOL1-G0, but absent in the mice co-expressing HIV and APOL1-G2. Mortality and renal function tests were not significantly different between groups. APOL1-G0 expressed in podocytes may have a protective function against podocyte loss or injury when exposed to an environmental stressor. This was absent with APOL1-G2 expression, suggesting APOL1-G2 may have lost this protective function.

Klíčová slova:

Glomeruli – Chronic kidney disease – Kidneys – Mouse models – Renal system – Animal models of disease – Genetically modified animals


Zdroje

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