Evaluation of soybean genotypes for reaction to natural field infection by Cercospora species causing purple seed stain

Autoři: Shuxian Li aff001;  Gabe Sciumbato aff002;  Debbie Boykin aff003;  Grover Shannon aff004;  Pengyin Chen aff004
Působiště autorů: United States Department of Agriculture, Agricultural Research Service (USDA, ARS), Crop Genetics Research Unit, Stoneville, Mississippi, United States of America aff001;  Mississippi State University, Delta Research and Extension Center, Stoneville, Mississippi, United States of America aff002;  USDA, ARS, Stoneville, Mississippi, United States of America aff003;  Division of Plant Sciences, University of Missouri, Portageville, Missouri, United States of America aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222673


Purple seed stain (PSS) of soybean (Glycine max (L.) Merr.) is a prevalent seed disease. It results in poor seed quality and reduced seed lot market grade, and thus undermines value of soybean worldwide. The objectives of this research were to evaluate the reaction of selected soybean genotypes collected from 15 countries representing maturity groups (MGs) III, IV, and V to PSS, and to identify new sources of resistance to PSS based on three years of evaluation of natural field infection by Cercospora spp. in the Mississippi Delta of the U. S. In this study, 42 soybean genotypes were evaluated in 2010, 2011, and 2012. Seventeen lines including six MG III (PI 88490, PI 504488, PI 417361, PI 548298, PI 437482, and PI 578486), seven MG IV (PI 404173, PI 346308, PI 355070, PI 416779, PI 80479, PI 346307, and PI 264555), and four MG V (PI 417567, PI 417420, PI 381659, and PI 407749) genotypes had significantly lower percent seed infection by Cercospora spp. than the susceptible checks and other genotypes evaluated (P ≤ 0.05). These genotypes of soybean can be used in developing soybean cultivars or germplasm lines with resistance to PSS and for genetic mapping of PSS resistance genes. In addition, among these 17 lines with different levels of resistance to PSS, nine soybean genotypes (PI 417361, PI 504488, PI 88490, PI 346308, PI 416779, PI 417567, PI 381659, PI 417567, and PI 407749) were previously reported as resistant to Phomopsis seed decay. Therefore, they could be useful in breeding programs to develop soybean cultivars with improved resistance to both seed diseases.

Klíčová slova:

Fungal pathogens – Humidity – Meteorology – Plant breeding – Plant pathogens – Seed germination – Seeds – Soybean


1. Pathan MA, Sinclair JB, McClary RD. Effects of Cercospora kikuchii on soybean seed germination and quality. Plant Dis. 1989; 73: 720–723.

2. Schuh W. Cercospora blight, leaf spot, and purple seed stain. In: Hartman GL, Sinclair JB, Rupe JC, editors. Compendium of soybean diseases. APS Press, Minnesota, USA; 1999. pp. 17–18.

3. Li S, Chen P, Zhang B, Shannon G. Research on purple seed stain of soybean: germplasm screening and genetic resistance. In: Melanie W, editor. Germplasm Characteristics, Diversity and Preservation. Nova Science Publisher; 2016. pp. 65–77.

4. Ward-Gauthier NA, Schneider RW, Chanda A, Silva EC, Price PP, Cai G. Cercospora leaf blight and purple seed stain. In: Hartman GL, Rupe JC, Sikora EJ, Domier LL, Davis JA, Steffey KL, editors. Compendium of soybean diseases and pests. APS Press. Minnesota, USA; 2015. pp 37–41.

5. Lee JH, Hwang S-R, Lee Y-H, Kim K, Cho KM, Lee YB. Chang occurring in compositions and antioxidant properties of healthy soybean seeds [Glycine max (L.) Merr.] and soybean seeds diseased by Phomopsis longicolla and Cercospora kikuchii fungal pathogens. Food Chem. 2015; 185: 205–211. doi: 10.1016/j.foodchem.2015.03.139 25952859

6. Wilcox JR, Abney TS. Effects of Cercospora kikuchii on soybeans. Phytopathology 1973; 63: 796–797.

7. Roy KW, Abney TS. Purple seed stain of soybeans. Phytopathology 1976; 66: 1045–1049.

8. Walters JA. Soybean leaf blight caused by Cercospora kikuchii. Plant Dis. 1980; 64: 961–962.

9. Yeh CC, Sinclair JB. Effect of Cercospora kikuchii on soybean seed germination and its interaction with Phomopsis sp. Phytopathol. Z. 1982; 105:265–270.

10. Suzuki K. Studies on the cause of “shihan” of soybean. Chosen Nakaiho 1921; 16: 24–28. (In Japanese).

11. Gardener MW. Indiana plant diseases, 1924. Proc. Indiana Acad. Sci. 1926; 35: 237–257.

12. Lehman SG. Soybean diseases. Fiftieth Annual Report of the North Carolina Agric. Expt. Sta., Raleigh; 1928.

13. Grau CR, Dorrance AE, Bond J, Russin JS. Fungal Diseases. In: Boerma HR, Specht JF, editors. Soybeans: Improvement, production, and uses. 3rd ed. Agron.Monogr. 16. American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Madison, Wisconsin, USA; 2004. pp. 679–763.

14. Matsumoto T, Tomoyasu R. Studies on the purple speck of soybean seed. Ann. Phytopath. Soc. Japan; 1925; 1: 1–14.

15. Groenewald JZ, Nakashima C, Nishikawa J, Park JH, Jama AN., Groenewald M, et al. Species concepts in Cercospora: spotting the weeds among the roses. Stud. Mycol. 2013; 75:115–170. doi: 10.3114/sim0012 24014899

16. Cai G, Schneider RW. Vegetative compatibility groups in Cercospora kikuchii, the causal agent of Cercospora leaf blight and purple seed stain in soybean. Phytopathology 2005; 95: 257–261. doi: 10.1094/PHYTO-95-0257 18943118

17. Cai G, Schneider RW. Population structure of Cercospora kikuchii, the causal agent of Cercospora leaf blight and purple seed stain in soybean. Phytopathology 2008; 98: 823–829. doi: 10.1094/PHYTO-98-7-0823 18943259

18. Soares APG, Guillin EA, Borges LL, da Silva ACT, de Almeida AMR, Grijalba PE,et al. More Cercospora species infect soybeans across the Americas than meets the eye. PLoS ONE. 2015; 5;10(8):e0133495.

19. Albu S, Schneider RW, Price PP, Doyle VP. Cercospora cf. flagellaris and C. cf. sigesbeckiae are associated with Cercospora leaf blight and purple seed stain on soybean in North America. Phytopathology 2016; 106: 1376–1385. doi: 10.1094/PHYTO-12-15-0332-R 27183302

20. Kuyama S, Tamura T. A pigment of Cercospora kikuchii (Matsumoto et Tomoyasu). Cultivation of the fungus, isolation and purification of pigment. J. Am. Chem. Soc. 1957; 79: 5725–5726.

21. Assante G, Locci R, Camarda L, Merlini L, Nasini G. Screening of genus Cercospora for secondary metabolites. Phytochemistry 1977; 16: 243–347.

22. Lynch FJ, Geoghegan MJ. Production of cercosporin by Cercospora species. Tran. Br. Mycol. Soc. 1977; 69: 496–498.

23. Upchurch RG, Walker DC, Rollins JA, Ehrenshaft M, Daub ME. Mutants of Cercospora kikuchii altered in cercosporin synthesis and pathogenicity. Appl. Env. Microbio. 1991; 57: 2940–2945.

24. Schuh W. Influence of temperature and leaf wetness period on conidial germination in vitro and infection of Cercospora kikuchii on soybean. Phytopathology 1991; 81: 1315–1318.

25. Schuh W. Influence of interrupted dew periods, relative humidity, and light on disease severity and latent infections caused by Cercospora kikuchii on soybean. Phytopathology 1993; 83: 109–113.

26. Schuh W. Effect of pod development stage, temperature, and pod wetness duration on the incidence of purple seed stain of soybeans. Phytopathology 1992; 82: 446–451.

27. Almeida AMR, Saraiva OF, Farias JRB, Gaudencio CA, Torres E. Survival of pathogens on soybean debris under no-tillage and conventional tillage systems. Pesqui. Agropecu. Bras. 2001; 36:1231–1238.

28. TeKrony DM, Egli DB, Stucky RE, Loeffler TM. Effect of benomyl applications on soybean seedborne fungi, seed germination, and yield. Plant Dis. 1985; 69: 763–765.

29. Wilcox JR, Laviolette FA, Martin RJ. Heritability of purple seed stain resistance in soybeans. Crop Sci. 1975; 15: 525–526.

30. Ploper LD, Abney TS, Roy KW. Influence of soybean genotype on rate of seed maturation and its impact on seedborne fungi. Plant Dis. 1992; 76: 287–292.

31. Srisombun S, Supapornhemin P. Inheritance of soybean resistance to purple seed stain. Soybean Genet. Newsl. 1993; 20: 92–93.

32. Jackson EW, Fenn P, Chen P. Inheritance of resistance to purple seed stain caused by Cercospora kikuchii in PI 80837 soybean. Crop Sci. 2006; 46:1462–1466.

33. Jackson EW, Fenn P, Chen P. Genetic mapping of resistance to purple seed stain in PI 80837 soybean. J. Hered. 2008; 99: 319–322. doi: 10.1093/jhered/esm123 18283049

34. Alloatti J, Chen P, Zeng A, Li S, Rupe J, Florez-Palacios L, et al., Inheritance of and molecular markers for purple seed stain resistance in soybean. Euphytica 2015; 206:701–709.

35. Song Q, Hyten DL, Jia G, Quigley CV, Fickus EW, Nelson RL, et al. Fingerprinting soybean germplasm and its utility in genomic research. G3: Genes| Genomes| Genetics 2015; 5: 1999–2006. doi: 10.1534/g3.115.019000 26224783

36. Chang H, Lipka AE, Domier LL and Hartman GL. Characterization of disease resistance loci in the USDA soybean germplasm collection using genome-wide association studies. Phytopathology 2016; 106: 1139–1151. doi: 10.1094/PHYTO-01-16-0042-FI 27135674

37. Li S, Smith J, Nelson R. Resistance to Phomopsis seed decay identified in maturity group V soybean plant introductions. Crop Sci. 2011; 51: 2681–2688.

38. Li S, Rupe J, Chen P, Shannon G, Wrather A, Boykin D. Evaluation of diverse soybean germplasm for resistance to Phomopsis seed decay. Plant Dis. 2015; 99: 1517–1525. doi: 10.1094/PDIS-04-14-0429-RE 30695950

39. Li S. Development of a seedling inoculation technique for rapid evaluation of soybean for resistance to Phomopsis longicolla under controlled conditions. Plant Methods. 14:81, 2018. Available from: https://doi.org/10.1186/s13007-018-0348-x. 30214468

40. Alloatti J, Li S, Chen P, Jaureguy L, Smith SF, Florez-Palacios L, et al. Screening a diverse soybean germplasm collection for reaction to purple seed stain caused by Cercospora kikuchii. Plant Dis. 2015; 99: 1140–1146. doi: 10.1094/PDIS-09-14-0878-RE 30695935

41. Hartman GL, Rupe JC, Sikora EJ, Domier LL, Davis JA, Steffey KL. Compendium of soybean diseases and pests. American Phytopathological Society (APS) Press. Minnesota, USA; 2015.

42. Association of Official Seed Analysts: Rules for testing seeds. Assoc. Official Seed Analysts, Las Cruces, NM; 2001.

43. Fehr WR, Caviness CE, Burmood DT, Pennington JS. Stage of development descriptions for soybeans, Glycine max (L.). Merrill. Crop Sci. 1971; 11: 929–931.

44. Pollack FG. An annotated compilation of Cercospora name. Mycological Mem. 1987; 12: 1–212.

45. Wilcox JR, Abney TS, Frankenberger EM. Relationships between seedborne soybean fungi and altered photoperiod. Phytopathology 1985; 75: 797–800.

46. Jones JP. Purple stain of soybean seeds incited by several Cercospora species. Phytopathology 1959; 49: 430–432.

47. Cai G, Schneider RW, Padgett GB. Assessment of lineages of Cercospora kikuchii in Louisiana for aggressiveness and screening soybean cultivars for resistance to Cercospora leaf blight. Plant Dis. 2009; 93: 868–874. doi: 10.1094/PDIS-93-9-0868 30754540

48. Bewley JD, Bradford K, Hilhorst H, Nonogaki H. Seeds: Physiology of Development, Germination and Dormancy. Springer; 2012.

49. Han YS. Studies on purple spot of soybean. J. Agric. For. Nat. Chung Hsing Univ. Taiwan. 1959; 8:1–32.

50. Lehman SG. Purple seed stain of soybeans. North Carolina Agric. Expt. Sta., Bulletin 369, Raleigh; 1950.

51. Hepperly PR, Sinclair JB. Relationships among Cercospora kikuchii, other seed mycoflora, and germination of soybean in Puerto Rico and Illinois. Plant Dis. 1981; 65: 130–132.

52. Roy KW, Abney TS. Antagonism between Cercospora kikuchii and other seedborne fungi of soybeans. Phytopathology 1977; 67: 1062–1066.

53. Li S, Chen P, Hartman GL. Phomopsis seed decay. In: Hartman GL, Rupe JC, Sikora EJ, Domier LL, Davis JA, Steffey KL, editors. Compendium of soybean diseases and pests. APS Press. Minnesota, USA; 2015. pp. 47–48.

54. Littell R, Milliken G, Stroup W, Wolfinger R, Schabenberger O. SAS for Mixed Models. SAS Press; 2006.

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