Health assessment of Conolophus subcristatus, Conolophus pallidus, and C. subcristatus X Amblyrhynchus cristatus hybrid (Galápagos land iguanas)

Autoři: Gregory A. Lewbart aff001;  Colon J. Grijalva aff002;  Paul P. Calle aff005;  Karen Ingerman aff005;  Juan Pablo Muñoz-Pérez aff002;  Galo Quezada aff008;  Carlos A. Vera aff008;  Gabriele Gentile aff009;  Carlos A. Valle aff001
Působiště autorů: Universidad San Francisco de Quito USFQ, Colegio de Ciencias Biológicas y Ambientales, Casilla, Quito, Ecuador aff001;  Galápagos Science Center GSC, Av. Alsacio Northia, Isla San Cristobal, Galápagos, Ecuador aff002;  North Carolina State University College of Veterinary Medicine, William Moore Drive, Raleigh, NC, United States of America aff003;  Universidad San Francisco de Quito, Escuela de Medicina Veterinaria, Diego de Robles s/n y Pampite, Quito, Ecuador aff004;  Wildlife Conservation Society, Zoological Health Program, New York, New York, United States of America aff005;  University of the Sunshine Coast, Sippy Downs, Queensland, Australia aff006;  Fundación Equilibrio Azul, Machalilla, Ecuador aff007;  Dirección Parque Nacional Galápagos, Technical Biodiversity Research, Puerto Ayora, Galápagos, Ecuador aff008;  Department of Biology, Tor Vergata University, Via della Ricerca Scientifica, Rome, Italy aff009
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222884


The land iguanas, Conolophus pallidus and Conolophus subcristatu are large and charismatic lizards endemic to the Galápagos archipelago, but little information exists on their normal health parameters. The former is restricted to Santa Fe island, while C. subcristatus inhabits the islands of the central and western region of the archipelago. Both species are classified as vulnerable by the IUCN Red List of Threatened Species. As part of a population health assessment authorized by the Galápagos National Park, wild adult iguanas from three islands (North Seymour, South Plazas, and Santa Fe) were captured in July 2018. Data from a single C. subcristatus X Amblyrhynchus cristatus hybrid captured on South Plazas is also included. We analyzed blood samples drawn from 52 healthy wild adult land iguanas captured on three islands. An iSTAT portable blood analyzer was used to obtain values for pH, lactate, pO2, pCO2, HCO3-, sO2%, hematocrit, packed cell volume (PCV), hemoglobin Na, K, iCa, and glucose. Standard laboratory hematology techniques were employed for PCV determination; resulting values were also compared to the hematocrit values generated by the iSTAT. Body temperature, heart rate, respiratory rate, and body measurements were also recorded and compared to previously published data for the marine iguana (Amblyrhynchus cristatus), which shares a common ancestor with the land iguana. The data reported here provide preliminary baseline values that may be useful in comparisons between captive and wild populations, between wild populations, and in detecting changes in health status among Galápagos land iguanas affected by anthropogenic threats, climate change, or natural disturbances.

Klíčová slova:

Biochemistry – Blood – Body temperature – Heart rate – Hematology – Hemoglobin – Islands – Iguanas


1. Werner D. 1984. The Galapagos Land Iguanas (Conolophus): Natural history and conservation surveys. Natl Geogr Soc Res Rep 17:81–94.

2. Christian KA, Porter WP, Tracy R. 1986. Core Areas within the home ranges of Galapagos land iguanas, Conolophus pallidus. J Herpetol 20: 272–276.

3. World Conservation Monitoring Centre. Conolophus pallidus. 1996a. The IUCN Red List of Threatened Species: e.T5239A11121073.

4. World Conservation Monitoring Centre. 1996b. Conolophus subcristatus. The IUCN Red List of Threatened Species: e.T5240A11121212.

5. Geffre A, Friedricks K, Harr K, Concordet D, Trumel C, Braun J-P. 2009. Reference values: a review. Vet Clin Path 38:288–298.

6. Klaphake E, Gibbons PM, Sladky KK, Carpenter J. 2017. Reptiles. In: Exotic Animal Formulary, 5th Edition ( Carpenter J. and Marion C. eds.), Elsevier Saunders, St. Louis, MO.

7. Lewbart GA, Hirschfeld M, Muñoz J, Denkinger J, Vinueza L, Brothers JR, García J, Lohmann KJ. 2015. Blood gasses, biochemistry, and hematology of marine iguanas (Amblyrhynchus cristatus). Conserv Physiol 3(1): cov034; doi: 10.1093/conphys/cov034 27293719

8. Dallwig RK, Paul-Murphy J, Thomas C, Medlin S, Vaughan C, Sullivan L, Sladky KK, Ramirez O, Herrera G. 2011. Hematology and clinical chemistry values of free-ranging basilisk lizards (Basiliscus plumifrons) in Costa Rica. J Zoo Wild Med 42(2):205–213.

9. James SB, Iverson V, Raphael B. 2006. Health assessment of Allen Cays rock iguana, Cyclura cychlura inornata. J Herpetol Med Surg 16:93–97.

10. Maria R, Ramer J, Reichard T, Tolson PJ, Christopher MM. 2007. Biochemical reference intervals and intestinal microflora of free-ranging Ricord's iguanas (Cyclura ricordii). J Zoo Wildl Med 38(3):414–9. doi: 10.1638/1042-7260(2007)38[414:BRIAIM]2.0.CO;2 17939350

11. Dennis PM, Bennet RA, Harr KE, Lock BA. 2001. Plasma concentration of ionized calcium in healthy iguanas. J Am Vet Med Assoc 219(3):326–8. doi: 10.2460/javma.2001.219.326 11497045

12. Divers SJ, Redmayne G, Aves EK. 1996. Haematological and biochemical values of 10 green iguanas (Iguana iguana). Vet Rec 138(9):203–5. doi: 10.1136/vr.138.9.203 8686152

13. Harr KE, Alleman AR, Dennis PM, Maxwell LK, Lock BA, Bennett RA, Jacobson E. 2001. Morphologic and cytochemical characteristics of blood cells and hematologic and plasma biochemical reference ranges in green iguanas. J Am Vet Med Assoc 218(6):915–21. doi: 10.2460/javma.2001.218.915 11294318

14. Hernandez-Divers SJ, Stahl SJ, Stedman NL, Hernandez-Divers SM, Schumacher J, Hanley CS, Wilson H, Vidyashankar AN, Zhao Y, Rumbeiha WK. 2005. Renal evaluation in the healthy green iguana (Iguana iguana): assessment of plasma biochemistry, glomerular filtration rate, and endoscopic biopsy. J Zoo Wildl Med 36(2):155–68. doi: 10.1638/03-120.1 17323554

15. Wagner RA, Wetzel R. 1999. Tissue and plasma enzyme activities in juvenile green iguanas. Am J Vet Res 60(2):201–203. 10048552

16. Peig J, Green AJ. 2009. New perspectives for estimating body condition from mass ⁄ length data: the scaled mass index as an alternative method. Oikos 118:1883–1891.

17. Peig J, Green AJ. 2010. The paradigm of body condition: a critical reappraisal of current methods based on mass and length. Funct Ecol 24:1323–1332

18. Warton DI, Duursma RA, Falster DS and Taskinen S. 2012. smatr 3—an R package for estimation and inference about allometric lines. Method Ecol Evolut 3(2):257–259.

19. R Development Team. 2018.

20. Harter TS, Reichert M, Brayner CJ, Milsom WK. 2015. Validation of the i-STAT and HemoCue systems for the analysis of blood parameters in the bar-headed goose, Anser indicus. Conserv Physiol 3: doi: 10.1093/conphys/cov021 27293706

21. Harter TS, Shartau RB, Baruner CJ, Farrell AP. 2014. Validation of the i-STAT system for the analysis of blood parameters in fish. Conserv Physiol 2(1):10. doi: 1093/conphys/cou037

22. Harter TS, Morrison PR, Mandelman JW, Rummer JL, Farrell AP, Brill RW, Baruner CJ. 2015. Validation of the i-STAT system for the analy sis of bllod gases and acid-base status in juvenile sandbar shark (Carcharhinus plumbeus). Conserv Physiol 3: doi: 10.1093/conphys/cov002 27293687

23. Alberts AC, Olivia ML, Worley MB, Telford SR, Morris PJ, Jannsen DL. 1998. The need for pre-release health screening in animal translocations: a case study of the Cuban iguana (Cyclura nubila). Anim Conserv 1:165–172.

24. Macleod A, Rodríguez A, Vences M, Orozco-terWengel P, Garcia C, Trillmich F, Gentile G, Caccone A, Quezeda G, Steinfartz S. 2015. Hybridization masks speciation in the evolutionary history of the Galápagos marine iguana. Proc R Soc B 282 (1809): 20150425. doi: 10.1098/rspb.2015.0425 26041359

25. Tzika AC, Rosa SF, Fabiani A, Snell HL, Snell HM, Marquez C, Tapia W, Rassmann K, Gentile G, Milinkovitch C. 2008. Population genetics of Galápagos land iguana (genus Conolophus) remnant populations. Mol Ecol 17:4943–4952. doi: 10.1111/j.1365-294X.2008.03967.x 19120985

26. Geist DJ, Snell H, Snell H, Goddard C, Kurz MD. 2014. A paleogeographic model of the Galápagos Islands and biogeographical and evolutionary implications In: The Galápagos: A natural laboratory for the earth sciences. Geophysical Monograph 204 (eds Harpp KS, Mittelstaedt E, d’Ozouville N, Graham DW), pp.145–166. New Jersey: John Wiley & Sons.

27. Wyles JS, Sarich VM. 1983. Are the Galápagos iguanas older than the Galápagos? Molecular evolution and colonization models for the archipelago. In Patterns of evolution in Galapagos organisms: 177–185. Bowman, RI, Berson M, Levinton AE (Eds). Am Assoc Adv Sci Pacific Div, San Francisco.

28. Rassmann K, Trillmich F, Tautz D. 1997. Hybridization between the Galápagos land and marine iguana (Conolophus subcristatus and Amblyrhynchus cristatus) on Plaza Sur. J Zool Lond 242:729–739.

29. Bartholomew & Lasiweski

30. Dessauer HC. 1970. Blood chemistry of reptiles: Physiological and evolutionary aspects, p. 1–72. In Gans C.and Parsons T. S.(eds.), Biology of the ReptiIia, Vol. 3. Academic Press, New York.

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2019 Číslo 10