Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis


Autoři: Maximilianos Kotsias aff001;  Athanasios Blanas aff002;  Sandra J. van Vliet aff002;  Martina Pirro aff003;  Daniel I. R. Spencer aff001;  Radoslaw P. Kozak aff001
Působiště autorů: Ludger Ltd., Culham Science Centre, Abingdon, Oxfordshire, England, United Kingdom aff001;  Amsterdam UMC, Vrije Universiteit Amsterdam, Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam, The Netherlands aff002;  Leiden University Medical Centre, Centre for Proteomics and Metabolomics, Leiden, The Netherlands aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223270

Souhrn

The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of N-glycans, in the effort to establish and standardize glycoproteomics technologies for quantitative glycan analysis from cultured cell lines. N-glycans from human gamma immunoglobulins (IgG), plasma and a pool of four cancer cell lines were released following three approaches and the performance of each method was evaluated.

Klíčová slova:

Blood plasma – Colorectal cancer – Consortia – Galactose – Glycosylation – HT29 cells – Hexoses – Mannose


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PLOS One


2019 Číslo 10

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