Development of an integrated fingerstick blood self-collection device for radiation countermeasures

Autoři: Jian Gu aff001;  Alan Norquist aff001;  Carla Brooks aff001;  Mikhail Repin aff002;  Sanjay Mukherjee aff002;  Jerome Lacombe aff001;  Jianing Yang aff001;  David J. Brenner aff002;  Sally Amundson aff002;  Frederic Zenhausern aff001
Působiště autorů: Center for Applied NanoBioscience and Medicine, The University of Arizona, College of Medicine, Phoenix, AZ, United States of America aff001;  Center for Radiological Research, Columbia University, Vagelos College of Physicians and Surgeons, New York, NY, United States of America aff002
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222951


We report the development of system for packaging critical components of the traditional collection kit to make an integrated fingerstick blood collector for self-collecting blood samples of 100 μl or more for radiation countermeasures. A miniaturized vacuum tube system (VacuStor system) has been developed to facilitate liquid reagent storage, simple operation and reduced sample contamination. Vacuum shelf life of the VacuStor tube has been analyzed by the ideal gas law and gas permeation theory, and multiple ways to extend vacuum shelf life beyond one year have been demonstrated, including low temperature storage, Parylene barrier coating and container vacuum bag sealing. Self-collection was also demonstrated by healthy donors without any previous fingerstick collection experience. The collected blood samples showed similar behavior in terms of gene expression and cytogenetic biodosimetry assays comparing to the traditionally collected samples. The integrated collector may alleviate the sample collection bottleneck for radiation countermeasures following a large-scale nuclear event, and may be useful in other applications with its self-collection and liquid reagent sample preprocessing capabilities.

Klíčová slova:

Blood – Blood volume – Capillaries – Gene expression – Permeability – RNA isolation – Specimen storage – Coatings


1. Coleman CN, Hrdina C, Bader JL, Norwood A, Hayhurst R, Forsha J, et al. Medical Response to a Radiologic/Nuclear Event: Integrated Plan From the Office of the Assistant Secretary for Preparedness and Response, Department of Health and Human Services. Ann Emerg Med. 2009 FEB;53(2):213–22. doi: 10.1016/j.annemergmed.2007.12.021 18387707

2. Grace MB, Moyer BR, Prasher J, Cliffer KD, Ramakrishnan N, Kaminski J, et al. Rapid Radiation Dose Assessment for Radiological Public Health Emergencies: Roles of Niaid and Barda. Health Phys. 2010 FEB;98(2):172–8. doi: 10.1097/01.HP.0000348001.60905.c0 20065680

3. Sullivan JM, Prasanna PGS, Grace MB, Wathen LK, Wallace RL, Koerner JF, et al. Assessment of Biodosimetry Methods for a Mass-Casualty Radiological Incident: Medical Response and Management Considerations. Health Phys. 2013 DEC;105(6):540–54. doi: 10.1097/HP.0b013e31829cf221 24162058

4. Repin M, Pampou S, Karan C, Brenner DJ, Garty G. RABiT-II: Implementation of a High-Throughput Micronucleus Biodosimetry Assay on Commercial Biotech Robotic Systems. Radiat Res. 2017 APR;187(4):492–8. doi: 10.1667/RR011CC.1 28231025

5. Repin M, Pampou S, Garty G, Brenner DJ. RABiT-II: A Fully-Automated Micronucleus Assay System with Shortened Time to Result. Radiat Res. 2019 MAR;191(3):232–6. doi: 10.1667/RR15215.1 30657421

6. Bush V, Cohen R. The evolution of evacuated blood collection tubes. Lab Med. 2003 APR;34(4):304–10.

7. Hoffmann J, Mark D, Lutz S, Zengerle R, von Stetten F. Pre-storage of liquid reagents in glass ampoules for DNA extraction on a fully integrated lab-on-a-chip cartridge. Lab Chip. 2010;10(11):1480–4. doi: 10.1039/b926139g 20480115

8. Chen D, Mauk M, Qiu X, Liu C, Kim J, Ramprasad S, et al. An integrated, self-contained microfluidic cassette for isolation, amplification, and detection of nucleic acids. Biomed Microdevices. 2010 AUG;12(4):705–19. doi: 10.1007/s10544-010-9423-4 20401537

9. Czurratis D, Beyl Y, Grimm A, Brettschneider T, Zinober S, Laermer F, et al. Liquids on-chip: direct storage and release employing micro-perforated vapor barrier films. Lab Chip. 2015;15(13):2887–95. doi: 10.1039/c5lc00510h 26038101

10. DuPont. Design Handbook for DuPont Engineering Polymers, Module 1: General Design Principles. 1992.

11. Li CG, Dangol M, Lee CY, Jang M, Jung H. A self-powered one-touch blood extraction system: a novel polymer-capped hollow microneedle integrated with a pre-vacuum actuator. Lab Chip. 2015;15(2):382–90. doi: 10.1039/c4lc00937a 25352059

12. MacNutt MJ, Sheel AW. Performance of evacuated blood collection tubes at high altitude. High Alt Med Biol. 2008;9(3):235–7. doi: 10.1089/ham.2008.1027 18800961

13. VANAMERONGEN GJ. The Permeability of Different Rubbers to Gases and its Relation to Diffusivity and Solubility. J Appl Phys. 1946;17(11):972–85.

14. McKeen LW. Permeability Properties of Plastics and Elastomers, 3rd Edition. NORWICH, NY: WILLIAM ANDREW INC; 2012.

15. Sawano S, Naka K, Werber A, Zappe H, Konishi S. Sealing method of PDMS as elastic material for MEMS. Mems 2008: 21st Ieee International Conference on Micro Electro Mechanical Systems, Technical Digest. 2008.

16. Hirshfield S, Teran RA, Downing MJ Jr, Chiasson MA, Tieu HV, Dize L, et al. Quantification of HIV-1 RNA Among Men Who Have Sex With Men Using an At-Home Self-Collected Dried Blood Spot Specimen: Feasibility Study. JMIR Public Health Surveill. 2018 Nov 1;4(4):e10847. doi: 10.2196/10847 30389648

17. Shah RF, Gupta RM. Video instruction is more effective than written instruction in improving inhaler technique. Pulm Pharmacol Ther. 2017 OCT;46:16–9. doi: 10.1016/j.pupt.2017.08.005 28797611

18. Paul S, Amundson SA. Development of gene expression signatures for practical radiation biodosimetry. Int J Radiat Oncol Biol Phys. 2008 JUL 15;71(4):1236–44. doi: 10.1016/j.ijrobp.2008.03.043 18572087

19. Xu L, Lee H, Jetta D, Oh KW. Vacuum-driven power-free microfluidics utilizing the gas solubility or permeability of polydimethylsiloxane (PDMS). Lab Chip. 2015;15(20):3962–79. doi: 10.1039/c5lc00716j 26329518

Článek vyšel v časopise


2019 Číslo 10