Development of an integrated fingerstick blood self-collection device for radiation countermeasures


Autoři: Jian Gu aff001;  Alan Norquist aff001;  Carla Brooks aff001;  Mikhail Repin aff002;  Sanjay Mukherjee aff002;  Jerome Lacombe aff001;  Jianing Yang aff001;  David J. Brenner aff002;  Sally Amundson aff002;  Frederic Zenhausern aff001
Působiště autorů: Center for Applied NanoBioscience and Medicine, The University of Arizona, College of Medicine, Phoenix, AZ, United States of America aff001;  Center for Radiological Research, Columbia University, Vagelos College of Physicians and Surgeons, New York, NY, United States of America aff002
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222951

Souhrn

We report the development of system for packaging critical components of the traditional collection kit to make an integrated fingerstick blood collector for self-collecting blood samples of 100 μl or more for radiation countermeasures. A miniaturized vacuum tube system (VacuStor system) has been developed to facilitate liquid reagent storage, simple operation and reduced sample contamination. Vacuum shelf life of the VacuStor tube has been analyzed by the ideal gas law and gas permeation theory, and multiple ways to extend vacuum shelf life beyond one year have been demonstrated, including low temperature storage, Parylene barrier coating and container vacuum bag sealing. Self-collection was also demonstrated by healthy donors without any previous fingerstick collection experience. The collected blood samples showed similar behavior in terms of gene expression and cytogenetic biodosimetry assays comparing to the traditionally collected samples. The integrated collector may alleviate the sample collection bottleneck for radiation countermeasures following a large-scale nuclear event, and may be useful in other applications with its self-collection and liquid reagent sample preprocessing capabilities.

Klíčová slova:

Blood – Blood volume – Capillaries – Gene expression – Permeability – RNA isolation – Specimen storage – Coatings


Zdroje

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Článek vyšel v časopise

PLOS One


2019 Číslo 10