Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads


Autoři: Seung Joon Lee aff001;  Tae Seok Sim aff003;  Hyun Young Shin aff001;  Jungmin Lee aff001;  Min Young Kim aff001;  Joseph Sunoo aff002;  Jeong-Gun Lee aff003;  Kyungmoo Yea aff001;  Young Zoon Kim aff004;  Danny van Noort aff001;  Soo Kyung Park aff002;  Woon-Hae Kim aff001;  Kyun Woo Park aff006;  Minseok S. Kim aff001
Působiště autorů: Department of New Biology, DGIST, Daegu, Republic of Korea aff001;  CytoDx, Pangyo-ro, Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea aff002;  Samsung Electronics, Ltd., Maetan3-dong, Youngtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea aff003;  Division of Neurooncology and Department of Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Republic of Korea aff004;  Division of Biotechnology, IFM, Linköping University, Linköping, Sweden aff005;  Daejeon Wellness Hospital, Beon-gil, Dongseo-daero, Daedeok-gu, Daejeon, Republic of Korea aff006;  Translational Responsive Medicine Center, DGIST, Daegu, Republic of Korea aff007
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223193

Souhrn

Microchips are widely used to separate circulating tumor cells (CTCs) from whole blood by virtues of sophisticated manipulation for microparticles. Here, we present a chip with an 8 μm high and 27.9 mm wide slit to capture cancer cells bound to 3 μm beads. Apart from a higher purity and recovery rate, the slit design allows for simplified fabrication, easy cell imaging, less clogging, lower chamber pressure and, therefore, higher throughput. The beads were conjugated with anti-epithelial cell adhesion molecules (anti-EpCAM) to selectively bind to breast cancer cells (MCF-7) used to spike the whole blood. The diameter of the cell-bead construct was in average 23.1 μm, making them separable from other cells in the blood. As a result, the cancer cells were separated from 5 mL of whole blood with a purity of 52.0% and a recovery rate of 91.1%, and also we confirmed that the device can be applicable to clinical samples of human breast cancer patients. The simple design with microslit, by eliminating any high-aspect ratio features, is expected to reduce possible defects on the chip and, therefore, more suitable for mass production without false separation outputs.

Klíčová slova:

Blood – Blood cells – Breast cancer – Flow rate – Fluid flow – Glass – Microfluidics – Microbeads


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