Validation of a simple extraction procedure for bisphenol A identification from human plasma

Autoři: Idha Arfianti Wiraagni aff001;  Mustafa Ali Mohd aff003;  Rusdi bin Abd Rashid aff004;  Didi Erwandi bin Mohamad Haron aff003
Působiště autorů: Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia aff001;  Department of Forensic Medicine and Medicolegal, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia aff002;  Shimadzu‐UM Center for Xenobiotics Studies (SUCXeS), Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia aff003;  Department of Psychological Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0221774


The general population is exposed to bisphenol A (BPA) orally, parenterally, transdermally, and environmentally as a result of the use of BPA in food packaging, plastics, and personal care products. The majority of the population nowadays (91–99%) has detectable levels of BPA inside their body. In this study, we successfully performed an inexpensive, rapid, and simple protein precipitation procedure for extraction of BPA from human plasma, followed by analysis by LC-MS/MS. This method was specifically developed for handling large numbers of samples with minimum cost and volume of sample. The developed method was accurate, precise, and reproducible for quantification of BPA from human plasma samples in the concentration range of 10–2000 ng/mL. The method was performed on samples from 150 healthy volunteers who were enrolled in the study. The mean of observed BPA level was 2.22 ± 9.91 ng/mL. Higher BPA levels were observed for females compare to that of males (p-value = 0.002), the BPA levels were higher in participants 33 years of age and older compared to those less than 33 years of age (p-value = 0.000), then the BPA levels higher in subjects with tap water as source of drinking (p-value = 0.005). This method may be valuable for general risk assessment of BPA for a large and varied population because of its efficiency and economical aspects.

Klíčová slova:

Blood – Blood plasma – Plasma proteins – Pollutants – Protein extraction – Quality control – Urine – Plastics


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