Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis

Autoři: David Lynch aff001;  Paula M. O’Connor aff002;  Paul D. Cotter aff002;  Colin Hill aff003;  Des Field aff003;  Máire Begley aff001
Působiště autorů: Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland aff001;  Teagasc Food Research Centre, Moorepark, Fermoy, Cork, Ireland aff002;  APC Microbiome Ireland, University College Cork, Cork, Ireland aff003;  School of Microbiology, University College Cork, Cork, Ireland aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223541


One hundred human-derived coagulase negative staphylococci (CoNS) were screened for antimicrobial activity using agar-based deferred antagonism assays with a range of indicator bacteria. Based on the findings of the screen and subsequent well assays with cell free supernatants and whole cell extracts, one strain, designated CIT060, was selected for further investigation. It was identified as Staphylococcus capitis and herein we describe the purification and characterisation of the novel bacteriocin that the strain produces. This bacteriocin which we have named capidermicin was extracted from the cell-free supernatant of S. capitis CIT060 and purified to homogeneity using reversed-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric (MS) analysis revealed that the capidermicin peptide has a mass of 5,464 Da. Minimal inhibitory concentration (MIC) experiments showed that capidermicin was active in the micro-molar range against all the Gram-positive bacteria that were tested. Antimicrobial activity was retained over a range of pHs (2–11) and temperatures (10–121°C x 15 mins). The draft genome sequence of S. capitis CIT060 was determined and the genes predicted to be involved in the biosynthesis of capidermicin were identified. These genes included the predicted capidermicin precursor gene, and genes that are predicted to encode a membrane transporter, an immunity protein and a transcriptional regulator. Homology searches suggest that capidermicin is a novel member of the family of class II leaderless bacteriocins.

Klíčová slova:

Bacteria – Gene prediction – Matrix-assisted laser desorption ionization time-of-flight mass spectrometry – Proteases – Staphylococcus – Staphylococcus aureus – Staphylococcus epidermidis – Lactococcus lactis


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