Linoleic acid supplementation of cell culture media influences the phospholipid and lipid profiles of human reconstructed adipose tissue


Autoři: Marie-Ève Ouellette aff001;  Jean-Christophe Bérubé aff003;  Jean-Michel Bourget aff001;  Maud Vallée aff001;  Yohan Bossé aff003;  Julie Fradette aff001
Působiště autorů: Centre de Recherche en Organogenèse Expérimentale de l'Université Laval/LOEX, Division of Regenerative Medicine, CHU de Québec -Université Laval Research Center, Québec, QC, Canada aff001;  Department of Surgery, Faculty of Medicine, Université Laval, Québec, Canada aff002;  Centre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec, QC, Canada aff003;  Department of Molecular Medicine, Faculty of Medicine, Université Laval, Québec, Canada aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224228

Souhrn

Reconstructed human adipose tissues represent novel tools available to perform in vitro pharmaco-toxicological studies. We used adipose-derived human stromal/stem cells to reconstruct, using tissue engineering techniques, such an adipose tridimensional model. To determine to what extent the in vitro model is representative of its native counterpart, adipogenic differentiation, triglycerides accumulation and phospholipids profiles were analysed. Ingenuity Pathway Analysis software revealed pathways enriched with differentially-expressed genes between native and reconstructed human adipose tissues. Interestingly, genes related to fatty acid metabolism were downregulated in vitro, which could be explained in part by the insufficient amount of essential fatty acids provided by the fetal calf serum used for the culture. Indeed, the lipid profile of the reconstructed human adipose tissues indicated a particular lack of linoleic acid, which could interfere with physiological cell processes such as membrane trafficking, signaling and inflammatory responses. Supplementation in the culture medium was able to influence the lipid profile of the reconstructed human adipose tissues. This study demonstrates the possibility to directly modulate the phospholipid profile of reconstructed human adipose tissues. This reinforces its use as a relevant physiological or pathological model for further pharmacological and metabolic studies of human adipose tissue functions.

Klíčová slova:

Adipocytes – Adipose tissue – Fats – Fatty acids – Lipid analysis – Lipid metabolism – Lipids – Phospholipids


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2019 Číslo 10