Antinociceptive compounds and LC-DAD-ESIMSn profile from Dictyoloma vandellianum leaves

Autoři: Lucas Silva Abreu aff001;  Iura Muniz Alves aff003;  Renan Fernandes do Espírito Santo aff002;  Yuri Mangueira do Nascimento aff001;  César Augusto Gonçalves Dantas aff001;  Gisele Graça Leite dos Santos aff004;  Mireille Le Hyaric aff005;  Maria Lenise Silva Guedes aff006;  Marcelo Sobral da Silva aff001;  Cristiane Flora Villarreal aff002;  Eudes da Silva Velozo aff002;  Josean Fechine Tavares aff001
Působiště autorů: Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, Universidade Federal da Paraíba, João Pessoa, Brazil aff001;  Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, Brazil aff002;  Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil aff003;  Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil aff004;  Departamento de Química, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil aff005;  Instituto de Biologia, Universidade Federal da Bahia, Salvador, Brazil aff006
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article


Limonoids, quinolone alkaloids and chromones have been reported as constituents of Dictyoloma vandellianum Adr. Juss. (Rutaceae). Although those compounds are known for their biological activities, only the anti-inflammatory activity of chromones isolated from the underground parts has been evaluated. There are no studies of the pharmacological properties of the aerial parts of D. vandellianum. The present study was carried out to determine the phytochemical profile and antinociceptive activity of the methanol extract, fractions and isolated compounds of leaves of D. vandellianum. The phytochemical profile was performed by HLPC-DAD-ESIMSn and pure substances obtained were characterized by MS and NMR spectroscopy. The antinociceptive activity was assessed using the formalin assay in mice, and the motor function in the rotarod test. ME and all the fractions obtained from ME produced antinociceptive effects. Among them, the ethyl ether fraction was the most active. Data from HPLC-DAD-ESIMSn showed that the ethyl ether fraction presented 42 compounds. The major compounds isolated from this fraction—gallic acid, methyl gallate and 1,2,6-tri-O-galloyl-β-d-glucopyranose–were tested and produced antinociceptive effects. Gallic acid, methyl gallate and 1,2,6-tri-O-galloyl-β-d-glucopyranose at antinociceptive doses did not affect the motor performance in mice in the rotarod test. This work is the first report of the occurrence of gallotanins in D. vandellianum. In addition, the pharmacological study showed that D. vandellianum leaves present antinociceptive activity, probably induced by gallic acid, methyl gallate and 1,2,6-tri-O-galloyl-β-d-glucopyranose.

Klíčová slova:

Analgesics – Ethers – Inflammation – Intraperitoneal injections – Leaves – Phytochemicals – Phase determination – Nociception


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