Comparative characterization of bacterial communities in geese consuming of different proportions of ryegrass

Autoři: Baodi Guo aff001;  Dianhui Li aff001;  Beibei Zhou aff001;  Yong Jiang aff001;  Hao Bai aff002;  Yang Zhang aff001;  Qi Xu aff001;  Wenming Zhao aff001;  Guohong Chen aff001
Působiště autorů: Laboratory of Animal Genetics and Rearing and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, PR, China aff001;  Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Jiangsu Yangzhou, China aff002
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223445


Geese are extremely well-adapted to utilizing plant-derived roughage in their diet, so the grass must be added to commercial diets under intensive rearing systems. However, it is unclear whether the gut microbiota will change significantly when adding different proportions of ryegrass. In this study, 240 healthy male Yangzhou geese (28 days old) with similar body weights were randomly divided into four groups and fed different proportions grass (CK, whole commercial diets; EG1, ryegrass: commercial diets = 1.5:1; EG2, ryegrass: commercial diets = 2:1; EG3, ryegrass: commercial diets = 3:1) respectively. When the geese grew to 70 days old, their intestines were collected and high-throughput sequencing technology was performed to investigate the microbial diversity in the caecum of geese with different dietary supplements. There was no obvious change in the alpha diversity of gut microbiota of geese with ryegrass intake (P > 0.05) and the composition of dominant bacterium (including Bacteroidetes and Firmicutes) was also similar. However, the ratio between Firmicutes and Bacteroidetes was remarkably reduced with ryegrass intake (P < 0.05), and the relative abundance of 30 operational taxonomic units (OTUs) significantly differed. Additionally, the content of cellulose-degrading microbiota such as Ruminiclostridium and Ruminococcaceae UCG-010 were significantly increased in geese fed with increasing amounts of grass. Finally, the functional profiles of the goose gut microbiota were explored using the PICRUSt tool. Carbohydrate metabolism and amino acid metabolism were dominant metabolic pathways. Lipid metabolism was significantly increased in EG3 compared that in the CK group (P < 0.05). Interestingly, Turicibacter and Parasutterella may have affected abdominal fat deposition as grass intake increased. Taken together, although the diversity of bacterial communities was similar in geese fed with different proportions of ryegrass, cellulose-degrading microbiota (Ruminiclostridium and Ruminococcaceae UCG-010) were abundant and the lipid metabolic pathway was enriched, which may reduce abdominal fat accumulation in high-ryegrass fed geese.

Klíčová slova:

Bacteria – Diet – Fats – Grasses – Gut bacteria – Microbiome – Ruminococcus – Ryegrass


1. Niu Q, Li P, Hao S, Zhang Y, Kim SW, Li H, et al. Dynamic distribution of the gut microbiota and the relationship with apparent crude fiber digestibility and growth stages in pigs. Scientific Reports. 2015;5(9938):9938.

2. Benson AK, Kelly SA, Legge R, Ma F, Low SJ, Kim J, et al. Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors. Proceedings of the National Academy of Sciences of the United States of America. 2010;107(44):18933–8. doi: 10.1073/pnas.1007028107 WOS:000283749000038. 20937875

3. Everard A, Lazarevic V, Gaã¯A N, Johansson M, Stã¥Hlman M, Backhed F, et al. Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity. Isme Journal. 2014;8(10):2116–30. doi: 10.1038/ismej.2014.45 24694712

4. Vangay P, Ward T, Gerber JS, Knights D. Antibiotics, pediatric dysbiosis, and disease. Cell Host & Microbe. 2015;17(5):553–64.

5. Xiaolei Z, Yonit BD, Laverde-Gomez JA, Bareket D, Sheridan PO, Duncan SH, et al. Unique Organization of Extracellular Amylases into Amylosomes in the Resistant Starch-Utilizing Human Colonic Firmicutes Bacterium Ruminococcus bromii. Mbio. 2015;6(5):e01058. doi: 10.1128/mBio.01058-15 26419877

6. Zhang CH, Zhang MH, Wang SY, Han RJ, Cao YF, Hua WY, et al. Interactions between gut microbiota, host genetics and diet relevant to development of metabolic syndromes in mice. Isme Journal. 2010;4(2):232–41. doi: 10.1038/ismej.2009.112 19865183

7. Schwartz S. A metagenomic study of diet-dependent interaction between gut microbiota and host in infants reveals differences in immune response. Genome Biology. 2012;13(4):r32. doi: 10.1186/gb-2012-13-4-r32 22546241

8. Wood KM, Salim H, Mcewen PL, Mandell IB, Miller SP, Swanson KC. The effect of corn or sorghum dried distillers grains plus solubles on growth performance and carcass characteristics of cross-bred beef steers. Animal Feed Science & Technology. 2011;165(1–2):23–30.

9. Xu Q, Yuan X, Gu T, Li Y, Dai W, Shen X, et al. Comparative characterization of bacterial communities in geese fed all-grass or high-grain diets. Plos One. 2017;12(10):e0185590. doi: 10.1371/journal.pone.0185590 28972993

10. Chen X, Liu X, Du Y, Wang B, Zhao N, Geng Z. Green forage and fattening duration differentially modulate cecal microbiome of Wanxi white geese. Plos One. 2018;13(9). doi: 10.1371/journal.pone.0204210 WOS:000445639700034. 30252869

11. Wang HY, Liu Y, Gong SM, Chen YS, Da-Qian HE. Effects of ryegrass amount on the weight gain,feed utilization rate and slaughter performance of Zhedong white geese. Acta Agriculturae Shanghai. 2015.

12. Kim JY, Kwon YM, Kim IS, Kim JA, Yu DY, Adhikari B, et al. Effects of the Brown Seaweed Laminaria japonica Supplementation on Serum Concentrations of IgG, Triglycerides, and Cholesterol, and Intestinal Microbiota Composition in Rats. Front Nutr. 2018;5:23. doi: 10.3389/fnut.2018.00023 29707542

13. Wen C, Yan W, Sun C, Ji C, Zhou Q, Zhang D, et al. The gut microbiota is largely independent of host genetics in regulating fat deposition in chickens. The ISME journal. 2019. doi: 10.1038/s41396-019-0367-2 MEDLINE:30728470. 30728470

14. Paola MD, Filippo CD, Cavalieri D, Ramazzotti M, Poullet JB, Massart S, et al. PP90 IMPACT OF DIET IN SHAPING GUT MICROBIOTA REVEALED BY A COMPARATIVE STUDY IN CHILDREN FROM EUROPE AND RURAL AFRICA. Proceedings of the National Academy of Sciences of the United States of America. 2010.

15. Wu GD, Christian H, Kyle B, Ying-Yu C, Keilbaugh SA, Meenakshi B, et al. Linking long-term dietary patterns with gut microbial enterotypes. Science. 2011.

16. Carlotta DF, Duccio C, Monica DP, Matteo R, Jean Baptiste P, Sebastien M, et al. Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. Proceedings of the National Academy of Sciences of the United States of America. 2010.

17. Emmanuelle LC, Trine N, Junjie Q, Edi P, Falk H, Gwen F, et al. Richness of human gut microbiome correlates with metabolic markers. Nature. 2013.

18. Zeng B, Han S, Wang P, Wen B, Jian W, Guo W, et al. The bacterial communities associated with fecal types and body weight of rex rabbits. Scientific Reports. 2015;5(9342):9342.

19. Junhua L, Tingting X, Weiyun Z, Shengyong M. High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats. British Journal of Nutrition. 2014;112(3):416–27. doi: 10.1017/S0007114514000993 24846282

20. Hyeun Bum K, Klaudyna B, White BA, Singer RS, Srinand S, Jin TZ, et al. Microbial shifts in the swine distal gut in response to the treatment with antimicrobial growth promoter, tylosin. Proceedings of the National Academy of Sciences of the United States of America. 2012;109(38):15485. doi: 10.1073/pnas.1205147109 22955886

21. Ley RE, Lozupone CA, Hamady M, Knight R, Gordon, I. J. Worlds within worlds: Evolution of the vertebrate gut microbiota. Nature Reviews Microbiology. 2008;6(10):776–88. doi: 10.1038/nrmicro1978 18794915

22. Velasco-Galilea M, Piles M, Viñas M, Rafel O, González-Rodríguez O, Guivernau M, et al. Rabbit Microbiota Changes Throughout the Intestinal Tract. Frontiers in Microbiology.

23. Song C, Wang B, Tan J, Zhu L, Lou D, Cen X. Comparative analysis of the gut microbiota of black bears in China using high-throughput sequencing. Molecular Genetics & Genomics. 2017;292(2):407–14.

24. Berry D. The emerging view of Firmicutes as key fibre degraders in the human gut. Environmental Microbiology. 2016;18(7):2081–3. doi: 10.1111/1462-2920.13225 26842002

25. Okazaki Y, Sekita A, Chiji H, Kato N. Consumption of lily bulb modulates fecal ratios of firmicutes and bacteroidetes phyla in rats fed a high-fat diet. Food Science & Biotechnology. 2016;25(1 Supplement):153–6.

26. Kaoutari AE, Armougom F, Gordon JI, Raoult D, Henrissat B. The abundance and variety of carbohydrate-active enzymes in the human gut microbiota. Nature Reviews Microbiology. 2013;11(7):497–504. doi: 10.1038/nrmicro3050 23748339

27. Ley RE, Turnbaugh PJ, Samuel K, Gordon JI. Microbial ecology: human gut microbes associated with obesity. Nature. 2006;444(7122):1022–3. doi: 10.1038/4441022a 17183309

28. Elie J, Itzhak M. Composition and similarity of bovine rumen microbiota across individual animals. Plos One. 2012;7(3):e33306. doi: 10.1371/journal.pone.0033306 22432013

29. Ravachol J, Borne R, Meynial-Salles I, Soucaille P, Pagès S, Tardif C, et al. Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum. Biotechnology for Biofuels. 2015;8(1):114.

30. Gosalbes MJ, Và zquez-Castellanos JF, Angebault C, Woerther PL, Ruppã© E, Ferrãºs ML, et al. Carriage of Enterobacteria Producing Extended-Spectrum Î2-Lactamases and Composition of the Gut Microbiota in an Amerindian Community. Antimicrob Agents Chemother. 2015;60(1):507–14. doi: 10.1128/AAC.01528-15 26552974

31. David LA, Maurice CF, Carmody RN, Gootenberg DB, Button JE, Wolfe BE, et al. Diet rapidly and reproducibly alters the human gut microbiome. Nature. 2014;505(7484):559. doi: 10.1038/nature12820 24336217

32. Gagen EJ, Padmanabha J, Denman SE, Mcsweeney CS. Hydrogenotrophic culture enrichment reveals rumen Lachnospiraceae and Ruminococcaceae acetogens and hydrogen-responsive Bacteroidetes from pasture-fed cattle. Fems Microbiology Letters. 2015;362(14).

33. Etxeberria U, Arias N, Boqué N, Macarulla MT, Portillo MP, Martínez JA, et al. Reshaping faecal gut microbiota composition by the intake of trans-resveratrol and quercetin in high-fat sucrose diet-fed rats. Journal of Nutritional Biochemistry. 2015;26(6):651–60. doi: 10.1016/j.jnutbio.2015.01.002 25762527

34. Amandine E, Vladimir L, Nadia G, Maria J, Marcus S, Fredrik B, et al. Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity. Isme Journal. 2014;8(10):2116–30. doi: 10.1038/ismej.2014.45 24694712

35. Li P, Yang C, Yue R, Zhen Y, Zhuo Q, Piao J, et al. Modulation of the Fecal Microbiota in Sprague-Dawley Rats Using Genetically Modified and Isogenic Corn Lines. J Agric Food Chem. 2018;66(2):551–61. doi: 10.1021/acs.jafc.7b05285 29264925

36. Noble EE, Hsu TM, Jones RB, Fodor AA, Goran MI, Kanoski SE. Early-Life Sugar Consumption Affects the Rat Microbiome Independently of Obesity. Journal of Nutrition. 2017;147(1):20–8. doi: 10.3945/jn.116.238816 27903830

37. Li M, Zhou H, Pan X, Xu T, Zhang Z, Zi X, et al. Cassava foliage affects the microbial diversity of Chinese indigenous geese caecum using 16S rRNA sequencing. Sci Rep. 2017;7:46837. doi: 10.1038/srep46837 28731455

Článek vyšel v časopise


2019 Číslo 10