Amphibians on the hotspot: Molecular biology and conservation in the South American Atlantic Rainforest


Autoři: Cesar R. L. Amaral aff001;  Anna C. S. Chaves aff001;  Vitor N. T. Borges Júnior aff001;  Filipe Pereira aff002;  Bruna M. Silva aff001;  Dayse A. Silva aff001;  António Amorim aff003;  Elizeu F. Carvalho aff001;  Carlos F. D. Rocha aff001
Působiště autorů: Departamento de Ecologia, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil aff001;  Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Porto, Portugal aff002;  Instituto de Patologia Molecular e Imunologia (IPATIMUP) / Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal aff003;  Faculdade de Ciências, Universidade do Porto, Porto, Portugal aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224320

Souhrn

Amphibians are the focus of a recent debate and public attention owing to the global decline in their populations worldwide. Amphibians are one of the most threatened and poorly known groups of vertebrates in several geographic areas, even though they play a central role in their own ecosystems. At different levels, amphibians make their contribution to proper ecosystem functioning. They act as regulators of the food web and nutrient cycling, and they also provide several valuable ecosystem services, e.g., as a food source and as animal models for lab research. In this sense, it seems clear that the maintenance of amphibian diversity should be one of the major goals for the several countries where their population decline is observed. However, we are still struggling with the very first step of this process, i.e., the correct identification of the amphibian species diversity. Over the past few decades, research on molecular identification of amphibians using DNA barcoding has encountered some difficulties related to high variability in the mitochondrial genome of amphibians, and a research gap is noticeable in the literature. We herein evaluated both COI and 16S rRNA mitochondrial genes for the molecular identification of frogs and tadpoles in a large fragment of the South American Atlantic Rainforest in Rio de Janeiro, Brazil. Our results suggest that both COI and 16S rRNA are informative markers for the molecular identification of the amphibian specimens with all specimens unambiguously identified at the species level. We also made publicly available 12 new sequences of Atlantic Rainforest amphibian species for the first time, and we discussed some conservation issues related to amphibians within the Atlantic Rainforest domains in the state of Rio de Janeiro, Brazil.

Klíčová slova:

Amphibians – Conservation science – DNA barcoding – Frogs – Rainforests – Ribosomal RNA – Species diversity – Tadpoles


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2019 Číslo 10