Genetic variability in landraces populations and the risk to lose genetic variation. The example of landrace ‘Kyperounda’ and its implications for ex situ conservation


Autoři: Angelos C. Kyratzis aff001;  Nikolaos Nikoloudakis aff002;  Andreas Katsiotis aff002
Působiště autorů: Department of Vegetable Crops, Agricultural Research Institute, Nicosia, Cyprus aff001;  Department of Agricultural Science, Biotechnology and Food Science, Cyprus University of Technology, Limassol, Cyprus aff002
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224255

Souhrn

Genetic characterization enhances the development of rational conservation strategies and the utilization of germplasm to plant breeding programs. In the present study, 19 microsatellite markers were employed to evaluate the genetic diversity and the genetic affiliations across 20 Cypriot durum wheat (Triticum turgidum L. subsp. durum) landraces, 13 landraces from the broader Mediterranean basin and 22 modern varieties. Cluster analysis depicted a clear separation among modern varieties and landraces, regardless of their origin. Landraces presented the highest genetic variation (average discriminating power of 0.89) and a high number of private alleles (131) was detected; underlying the unique genetic mark-up of this genepool. AMOVA revealed that the highest variability was detected within the landraces originating from Cyprus and landraces from the broader Mediterranean basin. The Cypriot landrace ‘Kyperounda’ was selected for further evaluation of its’ intra-genetic variation and it was determined that genetic diversity was higher in accessions conserved as sublines (He 0.643–0.731) than bulks (He 0.384–0.469). Bayesian analysis revealed substantial admixture within ‘Kyperounda’ accessions, depicted also by Principal Coordinate Analysis. The findings of the current manuscript emphasize that high intra-genetic diversity is retained when landraces are conserved as sublines in ex situ collections, while landraces that are conserved as bulks have a higher risk of bottleneck. Hence, a more exhausting diversity evaluation is needed in order to fully utilize landraces in breeding schemes and to prevent the loss of genetic variation.

Klíčová slova:

Alleles – Conservation genetics – Ears – Genetic polymorphism – Plant breeding – Population genetics – Wheat – Cyprus


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2019 Číslo 10