3D image analysis reveals differences of CD30 positive cells and network formation in reactive and malignant human lymphoid tissue (classical Hodgkin Lymphoma)


Autoři: Julia Liebers aff001;  Patrick Wurzel aff002;  Kerstin Bianca Reisinger aff001;  Martin-Leo Hansmann aff001
Působiště autorů: Reference and Consultant Center of Lymph Node and Lymphoma Pathology at Dr. Senckenberg Institute for Pathology, Frankfurt/Main, Hessen, Germany aff001;  Department of Molecular Bioinformatics, Johann Wolfgang Goethe-University Frankfurt/Main, Frankfurt/Main, Hessen, Germany aff002;  Johann-Wolfgang-Goethe-Universität Frankfurt am Main, Frankfurt Institute for Advanced Studies (FIAS), Frankfurt/Main, Hessen, Germany aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224156

Souhrn

Aims

The examination of histological sections is still the gold standard in diagnostic pathology. Important histopathological diagnostic criteria are nuclear shapes and chromatin distribution as well as nucleus-cytoplasm relation and immunohistochemical properties of surface and intracellular proteins. The aim of this investigation was to evaluate the benefits and drawbacks of three-dimensional imaging of CD30+ cells in classical Hodgkin Lymphoma (cHL) in comparison to CD30+ lymphoid cells in reactive lymphoid tissues.

Materials and results

Using immunoflourescence confocal microscopy and computer-based analysis, we compared CD30+ neoplastic cells in Nodular Sclerosis cHL (NScCHL), Mixed Cellularity cHL (MCcHL), with reactive CD30+ cells in Adenoids (AD) and Lymphadenitis (LAD). We confirmed that the percentage of CD30+ cell volume can be calculated. The amount in lymphadenitis was approx. 1.5%, in adenoids around 2%, in MCcHL up to 4,5% whereas the values for NScHL rose to more than 8% of the total cell cytoplasm. In addition, CD30+ tumour cells (HRS-cells) in cHL had larger volumes, and more protrusions compared to CD30+ reactive cells. Furthermore, the formation of large cell networks turned out to be a typical characteristic of NScHL.

Conclusion

In contrast to 2D histology, 3D laser scanning offers a visualisation of complete cells, their network interaction and spatial distribution in the tissue. The possibility to differentiate cells in regards to volume, surface, shape, and cluster formation enables a new view on further diagnostic and biological questions. 3D includes an increased amount of information as a basis of bioinformatical calculations.

Klíčová slova:

B cells – Cancer detection and diagnosis – Cell differentiation – Cytoplasm – Cytoplasmic staining – Histology – Image analysis – Hodgkin lymphoma


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2019 Číslo 10