Transcriptional foliar profile of the C3-CAM bromeliad Guzmania monostachia

Autoři: Helenice Mercier aff001;  Maria Aurineide Rodrigues aff001;  Sónia Cristina da Silva Andrade aff002;  Luiz Lehmann Coutinho aff003;  Bruno Nobuya Katayama Gobara aff001;  Alejandra Matiz aff001;  Paulo Tamaso Mioto aff004;  Ana Zangirolame Gonçalves aff001
Působiště autorů: Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil aff001;  Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil aff002;  Departamento de Zootecnia, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, São Paulo, Brazil aff003;  Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Santa Catarina, Brazil aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article


Guzmania monostachia is an epiphytic tank bromeliad that displays the inducible CAM photosynthesis under stressful conditions and had the highest stomata density in the leaf apex, while the base portion has the highest density of trichomes, which are specialized structures used to acquire water and nutrients from the tank solution. In order to correlate the genetic factors behind these morpho-physiological characteristics along the leaf blade of G. monostachia, a comparative transcriptome analysis was performed to identify the functional enriched pathways and unigenes that could play a role in the apical, middle and basal leaf portions. A total of 653 million reads were used for de novo transcriptome assembly, resulting in 48,051 annotated unigenes. Analysis of differentially expressed genes (DEGs) among distinct leaf regions revealed that 806 DEGs were upregulated in the apex compared to the middle portion, while 9685 DEGs were upregulated in the apex and 9784 DEGs were upregulated in the middle portions compared to the base. Our outcomes correlated some DEGs and identified unigenes with their physiological functions, mainly suggesting that the leaf apex was related to the regulation of stomatal movement, production of chlorophyll, cellular response to stress, and H2O2 catabolic process. In contrast, the middle portion showed DEGs associated with the transport of amino acids. Furthermore, DEGs from the leaf base were mainly correlated with responses to nutrients and nitrogen compounds, regulation of potassium ion import, response to water deprivation, and trichome branching, indicating that, at least in part, this leaf portion can replace some of the root functions of terrestrial plants. Therefore, possibly candidate unigenes and enriched pathways presented here could be prospected in future experimental work, opening new possibilities to bioengineer non-inducible CAM plants and/or improve the fertilization use efficiency by increasing leaf nutrient acquisition of crop plants.

Klíčová slova:

Cellular stress responses – Gene expression – Gene ontologies – Chlorophyll – Leaves – Sequence assembly tools – Transcriptome analysis – Trichomes


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2019 Číslo 10
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