Take one step backward to move forward: Assessment of genetic diversity and population structure of captive Asian woolly-necked storks (Ciconia episcopus)

Autoři: Kornsuang Jangtarwan aff001;  Tassika Koomgun aff001;  Tulyawat Prasongmaneerut aff001;  Ratchaphol Thongchum aff001;  Worapong Singchat aff001;  Panupong Tawichasri aff001;  Toshiharu Fukayama aff001;  Siwapech Sillapaprayoon aff001;  Ekaphan Kraichak aff003;  Narongrit Muangmai aff004;  Sudarath Baicharoen aff005;  Chainarong Punkong aff006;  Surin Peyachoknagul aff001;  Prateep Duengkae aff002;  Kornsorn Srikulnath aff001
Působiště autorů: Laboratory of Animal Cytogenetics and Comparative Genomics (ACCG), Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand aff001;  Special Research Unit for Wildlife Genomics, Department of Forest Biology, Faculty of Forestry, Kasetsart University, Chatuchak, Bangkok, Thailand aff002;  Department of Botany, Faculty of Science, Kasetsart University, Bangkok, Thailand aff003;  Department of Fishery Biology, Faculty of Fisheries, Kasetsart University, Bangkok, Thailand aff004;  Bureau of Research and Conservation, The Zoological Park Organization (ZPO), Bangkok, Thailand aff005;  Khao Kheow Open Zoo, Chonburi, Thailand aff006;  Center for Advanced Studies in Tropical Natural Resources (CASTNAR), National Research University-Kasetsart University (NRU-KU), Kasetsart University, Bangkok, Thailand aff007;  Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok, Thailand aff008;  Omics Center for Agriculture, Bioresources, Food and Health, Kasetsart University (OmiKU), Bangkok, Thailand aff009
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0223726


The fragmentation of habitats and hunting have impacted the Asian woolly-necked stork (Ciconia episcopus), leading to a serious risk of extinction in Thailand. Programs of active captive breeding, together with careful genetic monitoring, can play an important role in facilitating the creation of source populations with genetic variability to aid the recovery of endangered species. Here, the genetic diversity and population structure of 86 Asian woolly-necked storks from three captive breeding programs [Khao Kheow Open Zoo (KKOZ) comprising 68 individuals, Nakhon Ratchasima Zoo (NRZ) comprising 16 individuals, and Dusit Zoo (DSZ) comprising 2 individuals] were analyzed using 13 microsatellite loci, to aid effective conservation management. Inbreeding and an extremely low effective population size (Ne) were found in the KKOZ population, suggesting that deleterious genetic issues had resulted from multiple generations held in captivity. By contrast, a recent demographic bottleneck was observed in the population at NRZ, where the ratio of Ne to abundance (N) was greater than 1. Clustering analysis also showed that one subdivision of the KKOZ population shared allelic variability with the NRZ population. This suggests that genetic drift, with a possible recent and mixed origin, occurred in the initial NRZ population, indicating historical transfer between captivities. These captive stork populations require improved genetic variability and a greater population size, which could be achieved by choosing low-related individuals for future transfers to increase the adaptive potential of reintroduced populations. Forward-in-time simulations such as those described herein constitute the first step in establishing an appropriate source population using a scientifically managed perspective for an in situ and ex situ conservation program in Thailand.

Klíčová slova:

Conservation genetics – Genetic loci – Genetic polymorphism – Inbreeding – Microsatellite loci – Population genetics – Species diversity – Thailand


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