Does antibiotic use accelerate or retard cutaneous repair? A systematic review in animal models

Autoři: Luciana Schulthais Altoé aff001;  Raul Santos Alves aff001;  Mariáurea Matias Sarandy aff002;  Mônica Morais-Santos aff002;  Rômulo Dias Novaes aff003;  Reggiani Vilela Gonçalves aff002
Působiště autorů: Departament of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil aff001;  Departament of Animal Biology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil aff002;  Departament of Structural Biology, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223511



The presence of infections is one of the main factors that leads to delays in healing or non-closure of cutaneous wounds. Although the goal of antibiotic use is to treat or prevent infection, there is currently no agreement on the effectiveness of these products.


The aim of this study was to evaluate the efficacy of antibiotic use during the healing process of skin wounds in animal models not intentionally infected, as well as to analyze the advances and limitations of the studies carried out in this field.

Main methods

This systematic review was performed according to the PRISMA guidelines, using a structured search on the MedLine (PubMed) and Scopus platforms to retrieve studies published until August 29, 2018, 13:35p.m. The studies included were limited to those that used excision or incision wound models and that were not intentionally infected. The data for the animal models, antibiotic used, and the main results of the studies were extracted, and compared where possible. Bias analysis and methodological quality assessments were examined through the SYRCLE’s Risk of Bias tool.

Key findings

Twenty-seven studies were selected. Overall, the effects of the antibiotic on the wound decreased inflammatory cell infiltration and promoted an increased number of fibroblasts, extracellular matrix constituents, re-epithelialization and tissue strength. A great deal of important information about the methodology was not presented, such as: the statistical analysis used, the animal model (sex and age), antibiotic dosage, blinding and randomization of the animals chosen.


Based on the results found, we believe that antibiotic therapy can be considered a viable alternative for the treatment of cutaneous wounds. However, current evidence obtained from the methodological quality analysis points towards a high risk of bias. This is due to the incomplete characterization of the experimental design and treatment protocol, which compromises the reproducibility of the studies.

Klíčová slova:

Animal models – Antibiotics – Extracellular matrix – Fibroblasts – Inflammation – Silver – Systematic reviews – Tissue repair


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