Alteration of adipose tissue immune cell milieu towards the suppression of inflammation in high fat diet fed mice by flaxseed oil supplementation

Autoři: Samina Bashir aff001;  Yadhu Sharma aff001;  Deeba Jairajpuri aff002;  Faraz Rashid aff003;  Md. Nematullah aff001;  Farah Khan aff001
Působiště autorů: Department of Biochemistry, Jamia Hamdard, New Delhi, India aff001;  Department of Medical Biochemistry, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain aff002;  121 DHR, Udyog Vihar, Phase IV, Gurugram, Haryana, India aff003
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article


The present study evaluates the effect of flaxseed oil (FXO) supplementation on adipose tissue macrophages (ATM’s), E and D series resolvin (Rv) levels and adipose tissue inflammation. Male C57BL/6J mice were divided into five groups (n = 5): lean group (given standard chow diet), HFD group given high fat diet (approx. 18 weeks) till they developed insulin resistance and 4, 8 or 16 mg/kg group (HFD group later orally supplemented with 4, 8 or 16 mg/kg body weight flaxseed oil) for 4 weeks.The present study showed that FXO supplementation led to enhanced DHA, EPA, RvE1-E2, RvD2, RvD5- D6, IL-4, IL-10 and arginase 1 levels in ATMs together with altered immune cell infiltration and reduced NF-κB expression. The FXO supplementation suppresses immune cell infiltration into adipose tissue and alters adipose tissue macrophage phenotype towards the anti-inflammatory state via enhancement of E and D series resolvins, arginase 1 expression and anti-inflammatory cytokines level (IL-4 and IL-10.) leading to amelioration of insulin resistance in flaxseed oil supplemented HFD mice.

Klíčová slova:

Adipose tissue – Cytokines – Fats – Inflammation – Macrophages – Mouse models – Obesity – Oils


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2019 Číslo 10
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