Transcriptome sequencing reveals the effect of biochar improvement on the development of tobacco plants before and after topping

Autoři: Shen Yan aff001;  Zhengyang Niu aff001;  Haitao Yan aff001;  Aigai Zhang aff001;  Guoshun Liu aff001
Působiště autorů: Department of Tobacco cultivation, College of Tobacco Science, Henan Agricultural University, Zhengzhou, Henan Province, China aff001;  Henan Biochar Engineering Technology Research Center, Zhengzhou, Henan Province, China aff002;  Henan Biochar Technology Engineering Laboratory, Zhengzhou, Henan Province, China aff003;  Department of Microbiology, College of Agriculture and Life Science, Cornell University, Ithaca, NY, United States of America aff004
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224556


The application of biochar is one of the most useful methods for improving soil quality, which is of the utmost significance for the continuous production of crops. As there are no conclusive studies on the specific effects of biochar application on tobacco quality, this study aimed to improve the yield and quality of tobacco as a model crop for economic and genetic research in southern China, by such application. We used transcriptome sequencing to reveal the effects of applied biochar on tobacco development before and after topping. Our results showed that topping affected carbon and nitrogen metabolism, photosynthesis and secondary metabolism in the tobacco plants, while straw biochar-application to the soil resulted in amino acid and lipid synthesis; additionally, it affected secondary metabolism of the tobacco plants through carbon restoration and hormonal action, before and after topping. In addition to the new insights into the impact of biochar on crops, our findings provide a basis for biochar application measures in tobacco and other crops.

Klíčová slova:

Amino acid metabolism – Biosynthesis – Gene expression – Metabolic pathways – Metabolic processes – Nicotiana – Nitrogen metabolism – Straw


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2019 Číslo 10