Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen

Autoři: Giovani Trevisan aff001;  Leticia C. M. Linhares aff001;  Bret Crim aff001;  Poonam Dubey aff001;  Kent J. Schwartz aff001;  Eric R. Burrough aff001;  Rodger G. Main aff001;  Paul Sundberg aff002;  Mary Thurn aff003;  Paulo T. F. Lages aff003;  Cesar A. Corzo aff003;  Jerry Torrison aff003;  Jamie Henningson aff004;  Eric Herrman aff004;  Gregg A. Hanzlicek aff004;  Ram Raghavan aff004;  Douglas Marthaler aff004;  Jon Greseth aff005;  Travis Clement aff005;  Jane Christopher-Hennings aff005;  Daniel C. L. Linhares aff001
Působiště autorů: Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America aff001;  Swine Health Information Center, Ames, Iowa, United States of America aff002;  Veterinary Population Medicine, University of Minnesota, Saint Paul, Minnesota, United States of America aff003;  College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America aff004;  Veterinary & Biomedical Sciences Department, South Dakota State University, Brookings, South Dakota, United States of America aff005
Vyšlo v časopise: PLoS ONE 14(10)
Kategorie: Research Article


This project investigates the macroepidemiological aspects of porcine reproductive and respiratory syndrome virus (PRRSV) RNA detection by veterinary diagnostic laboratories (VDLs) for the period 2007 through 2018. Standardized submission data and PRRSV real-time reverse-transcriptase polymerase chain reaction (RT-qPCR) test results from porcine samples were retrieved from four VDLs representing 95% of all swine samples tested in NAHLN laboratories in the US. Anonymized data were retrieved and organized at the case level using SAS (SAS® Version 9.4, SAS® Institute, Inc., Cary, NC) with the use of PROC DATA, PROC MERGE, and PROC SQL scripts. The final aggregated and anonymized dataset comprised of 547,873 unique cases was uploaded to Power Business Intelligence—Power BI® (Microsoft Corporation, Redmond, Washington) to construct dynamic charts. The number of cases tested for PRRSV doubled from 2010 to 2018, with that increase mainly driven by samples typically used for monitoring purposes rather than diagnosis of disease. Apparent seasonal trends for the frequency of PRRSV detection were consistently observed with a higher percentage of positive cases occurring during fall or winter months and lower during summer months, perhaps due to increased testing associated with well-known seasonal occurrence of swine respiratory disease. PRRSV type 2, also known as North American genotype, accounted for 94.76% of all positive cases and was distributed across the US. PRRSV type 1, also known as European genotype, was geographically restricted and accounted for 2.15% of all positive cases. Co-detection of both strains accounted for 3.09% of the positive cases. Both oral fluid and processing fluid samples, had a rapid increase in the number of submissions soon after they were described in 2008 and 2017, respectively, suggesting rapid adoption of these specimens by the US swine industry for PRRSV monitoring in swine populations. As part of this project, a bio-informatics tool defined as Swine Disease Reporting System (SDRS) was developed. This tool has real-time capability to inform the US swine industry on the macroepidemiological aspects of PRRSV detection, and is easily adaptable for other analytes relevant to the swine industry.

Klíčová slova:

Data processing – Geographic distribution – Charts – Livestock – Swine – United States – Veterinary diagnostics – Veterinary diseases


1. Holtkamp DJ, Kliebenstein JB, Neumann EJ, Zimmerman JJ, Rotto HF, Yoder TK, et al. Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on United States pork producers. Journal of Swine Health and Production. 2013;21(2):72–84.

2. Hill H. Overview and history of mystery swine disease (swine infertility/respiratory syndrome). In Proceedings of the Mystery Swine Disease Committee Meeting, Livestock Conservation Institute. Denver, Colorado1990. p. 29–31.

3. Zimmerman JJ. Diseases of swine / edited by Jeffrey J. Zimmerman [and 5 others]. Eleventh edition. ed. Zimmerman JJ, editor: Wiley-Blackwell; 2019.

4. Wensvoort G, Terpstra C, Pol JM, ter Laak EA, Bloemraad M, de Kluyver EP, et al. Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q. 1991;13(3):121–30. doi: 10.1080/01652176.1991.9694296 1835211

5. Terpstra C, Wensvoort G, Pol JM. Experimental reproduction of porcine epidemic abortion and respiratory syndrome (mystery swine disease) by infection with Lelystad virus: Koch’s postulates fulfilled. Vet Q. 1991;13(3):131–6. doi: 10.1080/01652176.1991.9694297 1949539

6. Collins JE, Benfield DA, Christianson WT, Harris L, Hennings JC, Shaw DP, et al. Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J Vet Diagn Invest. 1992;4(2):117–26. doi: 10.1177/104063879200400201 1616975

7. Kikuti M, Vilalta C, Sanhueza J, Fioravante P, Corzo C, Geary E. Interpreting MSHMP`s Chart 1 for PRRS and PEDv. SHMP 2018|19.44: University of Minnesota; 5/3/2019.

8. Sanhueza J, Geary E, Kikuti M, Vilalta C, Fioravante P, Corzo C. MSHMP PRRS Prevalence Chart Description. SHMP 2018|19.45: University of Minnesota; 5/10/2019.

9. MSHMP. Dr. Bob Morrison`s Swine Health Monitoring Project. SHMP 2018|19.26; 12/28/2018. p. 6.

10. NASS. Quarterly Hogs and Pigs.; 12/20/2018.

11. Hueston WD, Walker KD. Macroepidemiological contributions to quantitative risk assessment. Rev Sci Tech. 1993;12(4):1197–201. doi: 10.20506/rst.12.4.749 8312621

12. Hurd H, Hueston W, editors. Macroepidemiology as it affects decision making in animal health. XVII World Buiatrics Congress/XXV American Association of Bovine Partitioners Conference; 1992; Saint Paul, Minnesota.

13. Snomed CT.; 2019

14. Lopez WA, Angulo J, Zimmerman JJ, Linhares DCL. Porcine reproductive and respiratory syndrome monitoring in breeding herds using processing fluids. Journal of Swine Health and Production. 2018;26(3):146–50.

15. LOINC.; 2019

16. Pork Checkoff. State ranking by hogs and pigs inventory—2017; 2018

17. Holtkamp DJ, Polson DD, Torremorell M. Terminology for classifying swine herds by porcine reproductive and respiratory syndrome virus status. Journal of Swine Health and Production. 2011;19(1):44–56.

18. Prickett J, Simer R, Christopher-Hennings J, Yoon KJ, Evans RB, Zimmerman JJ. Detection of Porcine reproductive and respiratory syndrome virus infection in porcine oral fluid samples: a longitudinal study under experimental conditions. J Vet Diagn Invest. 2008;20(2):156–63. doi: 10.1177/104063870802000203 18319427

19. Prickett JR, Kim W, Simer R, Yoon K-J, Zimmerman J. Oral-fluid samples for surveillance of commercial growing pigs for porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 infections. Journal of Swine Health and Production. 2008;16(2):86–91.

20. Ramirez A, Wang C, Prickett JR, Pogranichniy R, Yoon KJ, Main R, et al. Efficient surveillance of pig populations using oral fluids. Prev Vet Med. 2012;104(3–4):292–300. doi: 10.1016/j.prevetmed.2011.11.008 22154249

21. Rotolo ML, Sun YX, Wang C, Gimenez-Lirola L, Baum DH, Gauger PC, et al. Sampling guidelines for oral fluid-based surveys of group-housed animals. Veterinary Microbiology. 2017;209:20–9. doi: 10.1016/j.vetmic.2017.02.004 28284415

22. Bjustrom-Kraft J, Christopher-Hennings J, Daly R, Main R, Torrison J, Thurn M, et al. The use of oral fluid diagnostics in swine medicine. Journal os Swine Health and Production. 2018;26(5):262–9.

23. Lopez WA, Zimmerman JJ, Angulo J, Linhares DCL, editors. Processing fluids for detection of PRRS activity in neonates. 2017 ISU James D McKean Swine Disease Conference; 2017; Ames, IA.

24. Lopez W, Linhares D, editors. Processing fluids, blood serum, and tail blood swabs to detect PRRSV RNA and PCV2 DNA by PCR-Based assays. 2017 ISU James D McKean Swine Disease Conference; 2017; Ames, IA.

25. Dee S, Deen J, Rossow K, Wiese C, Otake S, Joo HS, et al. Mechanical transmission of porcine reproductive and respiratory syndrome virus throughout a coordinated sequence of events during cold weather. Can J Vet Res. 2002;66(4):232–9. 12418778

26. Dee S, Deen J, Rossow K, Weise C, Eliason R, Otake S, et al. Mechanical transmission of porcine reproductive and respiratory syndrome virus throughout a coordinated sequence of events during warm weather. Can J Vet Res. 2003;67(1):12–9. 12528824

27. Stevenson GW, Hoang H, Schwartz KJ, Burrough ER, Sun D, Madson D, et al. Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences. J Vet Diagn Invest. 2013;25(5):649–54. doi: 10.1177/1040638713501675 23963154

28. Wang L, Byrum B, Zhang Y. Detection and genetic characterization of deltacoronavirus in pigs, Ohio, USA, 2014. Emerg Infect Dis. 2014;20(7):1227–30. doi: 10.3201/eid2007.140296 24964136

29. Marthaler D, Jiang Y, Collins J, Rossow K. Complete Genome Sequence of Strain SDCV/USA/Illinois121/2014, a Porcine Deltacoronavirus from the United States. Genome Announc. 2014;2(2).

30. MSHMP. Morrison Swine Health Monitoring Project; 2018 [MSHMP history].

31. Dórea FC, Vial F. Animal health syndromic surveillance: a systematic literature review of the progress in the last 5 years (2011–2016). Vet Med (Auckl). 2016;7:157–70.

32. Mondoca E, Batista L, Cano J-P, Díaz E, Philips R, Polson D. General guidelines for porcine reproductive and respiratory syndrome regional control and elimination projects. Journal of Swine Health and Production. 2014.:84,8.

Článek vyšel v časopise


2019 Číslo 10
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