Identification of QTLs for resistance to maize rough dwarf disease using two connected RIL populations in maize

Autoři: Xintao Wang aff001;  Qing Yang aff001;  Ziju Dai aff001;  Yan Wang aff001;  Yingying Zhang aff001;  Baoquan Li aff001;  Wenming Zhao aff002;  Junjie Hao aff003
Působiště autorů: Crop Designing Center, Henan Academy of Agricultural Sciences, Zhengzhou, China aff001;  Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China aff002;  Plant Protection Institute, Henan Academy of Agricultural Sciences, Zhengzhou, China aff003
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226700


Maize rough dwarf disease (MRDD) is a significant viral disease caused by rice black-streaked dwarf virus (RBSDV) in China, which results in 30% yield losses in affected summer maize-growing areas. In this study, two connected recombinant inbred line (RIL) populations were constructed to elucidate the genetic basis of resistance during two crop seasons. Ten quantitative trait loci (QTLs) for resistance to MRDD were detected in the two RILs. Individual QTLs accounted for 4.97–23.37% of the phenotypic variance explained (PVE). The resistance QTL (qZD-MRDD8-1) with the largest effect was located in chromosome bin 8.03, representing 16.27–23.37% of the PVE across two environments. Interestingly, one pair of common significant QTLs was located in the similar region on chromosome 4 in both populations, accounting for 7.11–9.01% of the PVE in Zheng58×D863F (RIL-ZD) and 9.43–13.06% in Zheng58×ZS301 (RIL-ZZ). A total of five QTLs for MRDD resistance trait showed significant QTL-by-Environment interactions (QEI). Two candidate genes associated with resistance (GDSL-lipase and RPP13-like gene) which were higher expressed in resistant inbred line D863F than in susceptible inbred line Zheng58, were located in the physical intervals of the major QTLs on chromosomes 4 and 8, respectively. The identified QTLs will be studied further for application in marker-assisted breeding in maize genetic improvement of MRDD resistance.

Klíčová slova:

Genetic loci – Inbred strains – Leaves – Linkage mapping – Maize – Polymerase chain reaction – Quantitative trait loci – Veterinary diseases


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2019 Číslo 12