Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans


Autoři: Haruka Mori aff001;  Keita Ashida aff002;  Hisashi Shidara aff001;  Tatsuya Nikai aff001;  Kohji Hotta aff001;  Kotaro Oka aff001
Působiště autorů: Department of Bioscience and Informatics, Faculty of Science and Technology, Keio University, Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, Japan aff001;  Universal Biology Institute, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan aff002;  Department of Biological Sciences, Faculty of Science, Hokkaido University, Kita-ku, Sapporo, Hokkaido, Japan aff003;  Waseda Research Institute for Science and Engineering, Waseda University, Wakamatsucho, Shinjuku, Tokyo, Japan aff004;  Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan aff005
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226044

Souhrn

Animals change their behaviors in response to external stimuli, and numerous neurotransmitters are involved in these behavioral changes. In Caenorhabditis elegans, serotonin (5-HT) affects various behaviors such as inhibition of locomotion, stimulation of egg laying, and pharyngeal pumping. Previous research has shown that the neural activity of the RID interneuron increases when the worm moves forward, and the RID is necessary for sustaining forward locomotion. However, the relationship between 5-HT and neural activity of RID, and how it modulates the behavior of the worm has not been investigated. In this article, we reveal the relationship among 5-HT, RID activity, and the behavior of worms using a custom-made tracking and imaging system. We simultaneously measured the neural activity of the RID and behavior in worms with three conditions: mock animals, animals pre-exposed to 5-HT, and 5-HT receptor mod-1 mutants. As shown in previous research, the neural activity of the RID increased during the transition from backward to forward, whereas it decreased during the transition from forward to backward in mock animals. These changes in neural activity were not observed in animals pre-exposed to 5-HT and mod-1 mutants. Moreover, RID activity was correlated with the velocity of the worm in mock animals. However, this correlation was not observed in animals pre-exposed to 5-HT and mod-1 mutants. Our results demonstrate that 5-HT modulates the activity of the RID interneuron, and we infer that the RID plays a role in modulating forward locomotion by changing its activity through 5-HT.

Klíčová slova:

Animal behavior – Biological locomotion – Caenorhabditis elegans – Fluorescence imaging – Interneurons – Neuroimaging – Neurons – Serotonin


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Článek vyšel v časopise

PLOS One


2019 Číslo 12