Orbit image analysis machine learning software can be used for the histological quantification of acute ischemic stroke blood clots


Autoři: Seán Fitzgerald aff001;  Shunli Wang aff002;  Daying Dai aff002;  Dennis H. Murphree, Jr. aff005;  Abhay Pandit aff001;  Andrew Douglas aff001;  Asim Rizvi aff002;  Ramanathan Kadirvel aff002;  Michael Gilvarry aff006;  Ray McCarthy aff006;  Manuel Stritt aff007;  Matthew J. Gounis aff008;  Waleed Brinjikji aff002;  David F. Kallmes aff002;  Karen M. Doyle aff001
Působiště autorů: CÚRAM–Centre for Research in Medical Devices, National University of Ireland Galway, Galway, Ireland aff001;  Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America aff002;  Department of Physiology, National University of Ireland Galway, Galway, Ireland aff003;  Department of Pathology, Shanghai East Hospital, Tongji University, Shanghai, China aff004;  Department of Health Sciences Research, Mayo Clinic, Rochester, Minnesota, United States of America aff005;  Cerenovus, Ballybrit, Galway, Ireland aff006;  Orbit Image Analysis, Binningen, Switzerland aff007;  Department of Radiology, New England Center for Stroke Research, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America aff008
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225841

Souhrn

Our aim was to assess the utility of a novel machine learning software (Orbit Image Analysis) in the histological quantification of acute ischemic stroke (AIS) clots. We analyzed 50 AIS blood clots retrieved using mechanical thrombectomy procedures. Following H&E staining, quantification of clot components was performed by two different methods: a pathologist using a reference standard method (Adobe Photoshop CC) and an experienced researcher using Orbit Image Analysis. Following quantification, the clots were categorized into 3 types: RBC dominant (≥60% RBCs), Mixed and Fibrin dominant (≥60% Fibrin). Correlations between clot composition and Hounsfield Units density on Computed Tomography (CT) were assessed. There was a significant correlation between the components of clots as quantified by the Orbit Image Analysis algorithm and the reference standard approach (ρ = 0.944**, p < 0.001, n = 150). A significant relationship was found between clot composition (RBC-Rich, Mixed, Fibrin-Rich) and the presence of a Hyperdense artery sign using the algorithmic method (X2(2) = 6.712, p = 0.035*) but not using the reference standard method (X2(2) = 3.924, p = 0.141). Orbit Image Analysis machine learning software can be used for the histological quantification of AIS clots, reproducibly generating composition analyses similar to current reference standard methods.

Klíčová slova:

Computed axial tomography – Fibrin – Hematoxylin staining – Histology – Image analysis – Machine learning – Machine learning algorithms – Red blood cells


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2019 Číslo 12