Short-term treatment with a peroxisome proliferator-activated receptor α agonist influences plasma one-carbon metabolites and B-vitamin status in rats


Autoři: Vegard Lysne aff001;  Bodil Bjørndal aff002;  Mari Lausund Grinna aff002;  Øivind Midttun aff003;  Per Magne Ueland aff002;  Rolf Kristian Berge aff002;  Jutta Dierkes aff005;  Ottar Nygård aff001;  Elin Strand aff002
Působiště autorů: Centre for Nutrition, Department of Clinical Science, University of Bergen, Bergen, Norway aff001;  Department of Clinical Science, University of Bergen, Bergen, Norway aff002;  Bevital A/S, Bergen, Norway aff003;  Department of Heart Disease, Haukeland University Hospital, Bergen, Norway aff004;  Centre for Nutrition, Department of Clinical Medicine, University of Bergen, Bergen, Norway aff005;  Mohn Nutrition Research Laboratory, Centre for Nutrition, University of Bergen, Bergen, Norway aff006;  Laboratory Medicine and Pathology, Haukeland University Hospital, Bergen, Norway aff007
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226069

Souhrn

Introduction

Peroxisome proliferator-activated receptors (PPARs) have been suggested to be involved in the regulation of one-carbon metabolism. Previously we have reported effects on plasma concentrations of metabolites along these pathways as well as markers of B-vitamin status in rats following treatment with a pan-PPAR agonist. Here we aimed to investigate the effect on these metabolites after specific activation of the PPARα and PPARγ subtypes.

Methods

For a period of 12 days, Male Wistar rats (n = 20) were randomly allocated to receive treatment with the PPARα agonist WY-14.643 (n = 6), the PPARγ agonist rosiglitazone (n = 6) or placebo (n = 8). The animals were sacrificed under fasting conditions, and plasma concentration of metabolites were determined. Group differences were assessed by one-way ANOVA, and planned comparisons were performed for both active treatment groups towards the control group.

Results

Treatment with a PPARα agonist was associated with increased plasma concentrations of most biomarkers, with the most pronounced differences observed for betaine, dimethylglycine, glycine, nicotinamide, methylnicotinamide, pyridoxal and methylmalonic acid. Lower levels were observed for flavin mononucleotide. Fewer associations were observed after treatment with a PPARγ agonist, and the most notable was increased plasma serine.

Conclusion

Treatment with a PPARα agonist influenced plasma concentration of one-carbon metabolites and markers of B-vitamin status. This confirms previous findings, suggesting specific involvement of PPARα in the regulation of these metabolic pathways as well as the status of closely related B-vitamins.

Klíčová slova:

Blood plasma – Cobalamins – Glycine – Cholines – Metabolic pathways – Metabolites – Oxidation – Serine


Zdroje

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