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Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens


Autoři: Norfitriah Mohamed Sohaimi aff001;  Mohd Hair Bejo aff001;  Abdul Rahman Omar aff001;  Aini Ideris aff001;  Nurulfiza Mat Isa aff002
Působiště autorů: Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia aff001;  Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia aff002;  Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia aff003
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0225863

Souhrn

Fowl adenovirus (FAdV) is the causative agent of inclusion body hepatitis (IBH) in chickens with significant economic losses due to high mortality and poor production. It was objectives of the study to attenuate and determine the molecular characteristic of FAdV isolate (UPM1137) of Malaysia passages in primary chicken embryo liver (CEL) cells. The cytopathic effect (CPE) was recorded and the present of the virus was detected by polymerase chain reaction (PCR). Nucleotide and amino acid changes were determined and a phylogenetic tree was constructed. The pathogenicity and immunogenicity of the virus at passage 35 (CEL35) with virus titre of 106.7TCID50/mL was determined in day old specific pathogen free (SPF) chicks via oral or subcutaneous route of inoculation. The study demonstrated that the FAdV isolate was successfully propagated and attenuated in CEL cells up to 35th consecutive passages (CEL35) with delayed of CPE formation within 48 to 72 post inoculation (pi) from CEL20 onwards. The virus caused typical CPE with basophilic intranuclear inclusion bodies, refractile and clumping of cells. The virus is belong to serotype 8b with substitution of amino acid at position 44, 133 and 185 in L1 loop of hexon gene and in knob of fiber gene at position 348 and 360 at CEL35. It is non-pathogenic, but immunogenic in SPF chickens. It was concluded that the FAdV isolate was successfully attenuated in CEL cells with molecular changes in major capsid proteins which affect its infectivity in cell culture and SPF chickens.

Klíčová slova:

Adenoviruses – Amino acid sequence analysis – Antigens – DNA sequence analysis – Chickens – Nucleotide sequencing – Phylogenetic analysis – Polymerase chain reaction


Zdroje

1. Alvarado IR, Villegas P, El-Attrache J, Jensen E, Rosales G, Perozo F, et al. Genetic characterization, pathogenicity, and protection studies with an avian adenovirus isolate associated with inclusion body hepatitis. Avian Dis. 2007;51(1); 27–32. doi: 10.1637/0005-2086(2007)051[0027:GCPAPS]2.0.CO;2 17461263

2. Gomis S, Goodhope R, Ojkic D, Wilson P. Inclusion body hepatitis as a primary disease in broilers in Saskatchewan, Canada. Avian Dis. 2006;50(4); 550–555. doi: 10.1637/7577-040106R.1 17274293

3. Benko M, Harrach B, Both GW, Russell WC, Adair BM, Ádám E, et al. Family Adenoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, editors. Virus Taxonomy. Classification and Nomenclature of Viruses. The 8th Report of the International Committee on Taxonomy of Viruses. London: Elsevier/Academic Press. 2005; pp. 213–228.

4. Norfitriah MS, Hair-Bejo M, Majdi A. Pathogenicity of fowl adenovirus seroype 8b isolates of Malaysia in specific pathogen free chickens. J. Anim Vet Adv. 2019;18(3);78–83

5. Ojkic D, Martin E, Swinton J, Vaillancourt JP, Boulianne M et al. Genotyping of Canadian isolates of fowl adenoviruses. Avian Pathol. 2008;37(1); 95–100. doi: 10.1080/03079450701805324 18202956

6. Schonewille E, Singh A, Gobel TW, Gerner W, Saalmuller A et al. Fowl adenovirus serotype 4 causes depletion of B and T cells in lymphoid organs in specific pathogen free chickens following experimental infection. Vet Immunol Immunopathol. 2008;121; 130–139. doi: 10.1016/j.vetimm.2007.09.017 17996948

7. Singh A, Grewal GS, Maiti NK, Oberoi MS. Effect of fowl adenovirus-1 (IBH isolate) on humoral and cellular immune competency of broiler chicks. Comp Immunol Microbiol Infect Dis. 2006;29; 315–321. doi: 10.1016/j.cimid.2006.08.001 17045338

8. Hair-Bejo M. Inclusion body hepatitis in a flock of commercial broiler chickens. J Vet Malaysia. 2005;17; 23–26.

9. Juliana MA, Nurulfiza MI, Hair-Bejo M, Omar AR, Aini I. Molecular characterization of fowl adenoviruses isolated from inclusion body hepatitis outbreaks in commercial broiler chickens in Malaysia. Pertanika J Trop Agric Sci. 2014;37(4); 483–497.

10. Sohaimi NM, Bejo MH, Omar AR., Ideris AR, Isa NM. Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens. J Vet Sci. 2018;19(6); 759–770. doi: 10.4142/jvs.2018.19.6.759 30173491

11. Alexandera HS, Huber P, Cao J, Krell PJ, Nagy E. Growth characteristics of fowl adenovirus type 8 in a chicken hepatoma cell line. J Virol Methods. 1998;74; 9–14. doi: 10.1016/s0166-0934(98)00062-7 9763123

12. Mansoor MK, Hussain I, Arshad M, Muhammad G. Preparation and evaluation of chicken embryo-adapted fowl adenovirus serotype 4 vaccine in broiler chickens. Trop Anim Health Prod. 2011;43, 331–338. doi: 10.1007/s11250-010-9694-z 20878234

13. Barua S, Rai A. Cultivation of fowl adenovirus-4 in chick embryo liver cell culture and purification of the virus by ultracentrifugation. Indian J Comp Microbiol Immunol Infect Dis. 2003;24(2): 195–196.

14. Soumyalekshmi S, Ajith MK, Chandraprakash M. Isolation of fowl adenovirus in chicken embryo liver cell culture and its detection by hexon gene based PCR. Indian J Sci Res Tech. 2014;2(3); 33–36.

15. Paul G, Hess, MB (2003) U.S. Patent No. WO2003039593A1. Washington, DC: U.S. Patent and Trademark Office.

16. Ali S, Mahmood MS, Hussain I, Khan MN. Preparation and evaluation of lyophilized live attenuated vaccine of inclusion body hepatitis hydropericardium syndrome (IBH-HPS) against challenge in broiler chickens. Int J Agric Biol. 2015;17(3); 658–662.

17. Grgić H, Krell PJ, Nagy E. Comparison of fiber gene sequences of inclusion body hepatitis (IBH) and non-IBH strains of serotype 8 and 11 fowl adenoviruses. Virus Genes. 2014;48; 74–80. doi: 10.1007/s11262-013-0995-y 24142408

18. Norfitriah MS, Hair-Bejo M, Omar AR, Aini I, Nurulfiza MI. Molecular detection and pathogenicity of fowl adenovirus isolated from disease outbreak in commercial layer farm. Int. J. Agric. Sc. & Vet. Med. 2018;6; 73–84.

19. Alemnesh W, Hair-Bejo M, Aini I, Omar AR. Pathogenicity of fowl adenovirus in specific pathogen free chicken embryos. J Comp Pathol. 2012;146; 223–229. doi: 10.1016/j.jcpa.2011.05.001 21705014

20. Adair BM. Studies on the development of avian adenoviruses in cell cultures. Avian Pathol. 1978;7(4); 541–550. doi: 10.1080/03079457808418308 18770408

21. Reed LJ, Muench H. A simple method of estimating fifty per cent endpoints. Am J Hyg. 1938;27; 493–497.

22. Raue R, Hess M. Hexon based PCRs combined with restriction enzyme analysis for rapid detection and differentiation of fowl adenoviruses and egg drop syndrome virus. J Virol Methods. 1998;73; 211–217. doi: 10.1016/s0166-0934(98)00065-2 9766892

23. Grgić H, Yang D-H, Nagy E. Pathogenicity and complete genome sequence of fowl adenovirus serotype 8 isolate. Virus Res. 2011;156; 91–97. doi: 10.1016/j.virusres.2011.01.002 21237223

24. Nakamura T, Sato K, Hamada H. Reduction of natural adenovirus tropism to the liver by both ablation of fiber-coxsackievirus and adenovirus receptor interaction and use of replaceable short fiber. J Virol. 2003;77(4); 2512–2521. doi: 10.1128/JVI.77.4.2512-2521.2003 12551989

25. Tan PK, Michou A-I, Bergelson JM, Cotton M. Defining CAR as a cellular receptor for the adenovirus CELO using a genetic analysis of the two viral fibre proteins. J Gen Virol. 2001;82; 1465–1472. doi: 10.1099/0022-1317-82-6-1465 11369892

26. Pallister J, Wright PJ, Sheppard M. A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses. J Virol. 1996;70(8); 5115–5122. 8764019

27. Wang Z, Wang B, Lou J, Yan J, Gao L, Geng R, Yu B. Mutation in fiber gene of adenovirus 5 gene therapy vector decreases liver tropism. Int J Clin Exp Med. 2014;7(12); 4942–4950. 25663991

28. Schonewille E, Jaspers R, Paul G, Hess M. Specific pathogen free chickens vaccinated with a live FAdV-4 vaccine are fully protected against a severe challenge even in the absence of neutralizing antibodies. Avian Dis. 2010;54(2); 905–910. doi: 10.1637/8999-072309-Reg.1 20608537


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