Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family


Autoři: Gustavo Akira Toma aff001;  Renata Luiza Rosa de Moraes aff001;  Francisco de Menezes Cavalcante Sassi aff001;  Luiz Antonio Carlos Bertollo aff001;  Ezequiel Aguiar de Oliveira aff001;  Petr Rab aff003;  Alexandr Sember aff003;  Thomas Liehr aff004;  Terumi Hatanaka aff001;  Patrik Ferreira Viana aff005;  Manoela Maria Ferreira Marinho aff006;  Eliana Feldberg aff005;  Marcelo de Bello Cioffi aff001
Působiště autorů: Laboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, São Paulo, Brazil aff001;  Secretaria de Estado de Educação de Mato Grosso, Cuiabá, Mato Grosso, Brazil aff002;  Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic aff003;  Institute of Human Genetics, University Hospital Jena, Jena, Germany aff004;  Instituto Nacional de Pesquisas da Amazônia, Manaus, Amazonas, Brazil aff005;  Museu de Zoologia da Universidade de São Paulo, São Paulo, São Paulo, Brazil aff006
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226746

Souhrn

Lebiasinidae is a small fish family composed by miniature to small-sized fishes with few cytogenetic data (most of them limited to descriptions of diploid chromosome numbers), thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing, the first cytogenetic data for the red spotted tetra, Copeina guttata, including the standard karyotype, C-banding, repetitive DNA mapping by fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH), providing chromosomal patterns and novel insights into the karyotype differentiation of the family. Males and females share diploid chromosome number 2n = 42 and karyotype composed of 2 metacentric (m), 4 submetacentric (sm) and 36 subtelocentric to acrocentric (st-a) chromosomes. Blocks of constitutive heterochromatin were observed in the centromeric and interstitial regions of several chromosomes, in addition to a remarkably large distal block, heteromorphic in size, which fully corresponded with the 18S rDNA sites in the fourth chromosomal pair. This overlap was confirmed by 5S/18S rDNA dual-color FISH. On the other hand, 5S rDNA clusters were situated in the long and short arms of the 2nd and 15th pairs, respectively. No sex-linked karyotype differences were revealed by male/female CGH experiments. The genomic probes from other two lebiasinid species, Lebiasina melanoguttata and Pyrrhulina brevis, showed positive hybridization signals only in the NOR region in the genome of C. guttata. We demonstrated that karyotype diversification in lebiasinids was accompanied by a series of structural and numeric chromosome rearrangements of different types, including particularly fusions and fissions.

Klíčová slova:

Comparative genomics – Cytogenetics – Fluorescent in situ hybridization – Hybridization – Chromosome mapping – Chromosome pairs – Karyotypes – Sex chromosomes


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