Sequence analyses at mitochondrial and nuclear loci reveal a novel Theileria sp. and aid in the phylogenetic resolution of piroplasms from Australian marsupials and ticks

Autoři: Amanda D. Barbosa aff001;  Jill Austen aff001;  Timothy J. Portas aff003;  J. Anthony Friend aff004;  Liisa A. Ahlstrom aff005;  Charlotte L. Oskam aff001;  Una M. Ryan aff001;  Peter J. Irwin aff001
Působiště autorů: Vector- and Water-Borne Pathogen Research Group, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, WA, Australia aff001;  CAPES Foundation, Ministry of Education of Brazil, Brasília—DF, Brazil aff002;  Veterinary and Research Centre, Tidbinbilla Nature Reserve, Australian Capital Territory, Australia aff003;  Department of Biodiversity, Conservation and Attractions, Albany, WA, Australia aff004;  Bayer Australia Ltd, Pymble, Australia aff005
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article


The order Piroplasmida encompasses two main families: Babesiidae and Theileriidae, containing tick-borne pathogens of veterinary and medical importance worldwide. While only three genera (Babesia, Cytauxzoon and Theileria) comprising piroplasm parasites are currently recognised, phylogenetic studies at the 18S rRNA (18S) gene suggest that these organisms represent at least ten lineages, one of which comprises the relatively unique and highly diverse Theileria spp. from Australian marsupials and ticks. As an alternative to analysing 18S sequences alone, sequencing of mitochondrial genes has proven to be useful for the elucidation of evolutionary relationships amongst some groups of piroplasms. This research aimed to characterise piroplasms from Australian native mammals and ticks using multiple genetic markers (18S, cytochrome c, oxidase subunit III (cox3) and cytochrome B (cytB)) and microscopy. For this, nearly complete piroplasm-18S sequences were obtained from 32 animals belonging to six marsupial species: eastern bettong (Bettongia gaimardi), eastern quoll (Dasyurus viverrinus), eastern grey kangaroo (Macropus giganteus), swamp wallaby (Wallabia bicolor), quokka (Setonix brachyurus) and Gilbert’s potoroo (Potorous gilbertii). The organisms detected represented eight novel Theileria genotypes, which formed five sub-clades within the main marsupial clade containing previously reported Australian marsupial and tick-derived Theileria spp. A selection of both novel and previously described Australian piroplasms at the 18S were also successfully characterised, for the first time, at the cox3 and cytB loci, and corroborated the position of Australian native theilerias in a separate, well-supported clade. Analyses of the cox3 and cytB genes also aided in the taxonomic resolution within the clade of Australian Piroplasmida. Importantly, microscopy and molecular analysis at multiple loci led to the discovery of a unique piroplasm species that clustered with the Australian marsupial theilerias, for which we propose the name Theileria lupei n. sp.

Klíčová slova:

Babesia – DNA sequence analysis – Genetic loci – Phylogenetic analysis – Sequence alignment – Sequence analysis – Theileria – Marsupials


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