In Vitro detection of Chronic Wasting Disease (CWD) prions in semen and reproductive tissues of white tailed deer bucks (Odocoileus virginianus)


Autoři: Carlos Kramm aff001;  Ruben Gomez-Gutierrez aff001;  Claudio Soto aff001;  Glenn Telling aff004;  Tracy Nichols aff005;  Rodrigo Morales aff001
Působiště autorů: Department of Neurology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX, United States of America aff001;  Universidad de Los Andes, Facultad de Medicina, Las Condes, Santiago, Chile aff002;  Department of Cell Biology, University of Malaga, Malaga, Spain aff003;  Prion Research Center, Colorado State University, Fort Collins, CO, United States of America aff004;  Veterinary Services, APHIS, United States Department of Agriculture, Fort Collins, CO, United States of America aff005;  CIBQA, Universidad Bernardo OHiggins. Santiago, Chile aff006
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226560

Souhrn

Chronic Wasting Disease (CWD) is a prion disease affecting several cervid species. Among them, white-tailed deer (WTD) are of relevance due to their value in farming and game hunting. The exact events involved in CWD transmission in captive and wild animals are still unclear. An unexplored mechanism of CWD spread involves transmissions through germplasm, such as semen. Surprisingly, the presence and load of CWD prions in semen and male sexual tissues from WTD has not been explored. Here, we described the detection of CWD prions in semen and sexual tissues of WTD bucks utilizing the Protein Misfolding Cyclic Amplification (PMCA) technology. Samples were obtained post-mortem from farmed pre-clinical, CWD positive WTD bucks possessing polymorphisms at position 96 of the PRNP gene. Our results show that overall CWD detection in these samples had a sensitivity of 59.3%, with a specificity of 97.2%. The data indicate that the presence of CWD prions in male sexual organs and fluids is prevalent in late stage, pre-clinical, CWD-infected WTD (80%-100% of the animals depending on the sample type analyzed). Our findings reveal the presence of CWD prions in semen and sexual tissues of prion infected WTD bucks. Future studies will be necessary to determine whether sexual contact and/or artificial inseminations are plausible means of CWD transmission in susceptible animal species.

Klíčová slova:

Animal prion diseases – Blood – Deer – Chronic wasting disease – Prions – Semen – Testes – Epididymis


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2019 Číslo 12