Robust effect of metabolic syndrome on major metabolic pathways in the myocardium


Autoři: Maryam Karimi aff001;  Vasile I. Pavlov aff002;  Olivia Ziegler aff001;  Nivedita Sriram aff001;  Se-Young Yoon aff001;  Vahid Agbortoko aff001;  Stoiana Alexandrova aff003;  John Asara aff003;  Frank W. Sellke aff001;  Michael Sturek aff004;  Jun Feng aff001;  Boian S. Alexandrov aff005;  Anny Usheva aff001
Působiště autorů: Department of Surgery, Rhode Island Hospital, The Warren Alpert Medical School, Brown University, Providence, RI, United States of America aff001;  Icahn School of Medicine at Mount Sinai, New York, NY, United States of America aff002;  Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States of America aff003;  Indiana University, School of Medicine, Indianapolis, IN, United States of America aff004;  Los Alamos National Laboratory, Los Alamos, NM, United States of America aff005
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225857

Souhrn

Although the high-fat-diet-induced metabolic syndrome (MetS) is a precursor of human cardiac pathology, the myocardial metabolic state in MetS is far from clear. The discrepancies in metabolite handling between human and small animal models and the difficulties inherent in obtaining human tissue complicate the identification of the myocardium-specific metabolic response in patients. Here we use the large animal model of swine that develops the hallmark criteria of human MetS. Our comparative metabolomics together with transcriptomics and computational nonnegative matrix factorization (NMF) interpretation of the data exposes significant decline in metabolites related to the fatty acid oxidation, glycolysis, and pentose phosphate pathway. Behind the reversal lies decreased expression of enzymes that operate in the pathways. We showed that diminished glycogen deposition is a metabolic signature of MetS in the pig myocardium. The depletion of glycogen arises from disbalance in expression of genes that break down and synthesize glycogen. We show robust acetoacetate accumulation and activated expression of key enzymes in ketone body formation, catabolism and transporters, suggesting a shift in fuel utilization in MetS. A contrasting enrichment in O-GlcNAcylated proteins uncovers hexosamine pathway and O-GlcNAcase (OGA) expression involvement in the myocardial response to MetS. Although the hexosamine biosynthetic pathway (HBP) activity and the availability of the UDP-GlcNAc substrate in the MetS myocardium is low, the level of O-GlcNacylated proteins is high as the O-GlcNacase is significantly diminished. Our data support the perception of transcriptionally driven myocardial alterations in expression of standard fatty acids, glucose metabolism, glycogen, and ketone body related enzymes and subsequent paucity of their metabolite products in MetS. This aberrant energy metabolism in the MetS myocardium provide insight into the pathogenesis of CVD in MetS.

Klíčová slova:

Fatty acids – Gene expression – Glycogens – Glycolysis – Ketones – Metabolites – Myocardium – Swine


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Článek vyšel v časopise

PLOS One


2019 Číslo 12