Identification and characterization of compounds from Chrysosporium multifidum, a fungus with moderate antimicrobial activity isolated from Hermetia illucens gut microbiota

Autoři: Yesenia Correa aff001;  Billy Cabanillas aff001;  Valérie Jullian aff002;  Daniela Álvarez aff001;  Denis Castillo aff001;  Cédric Dufloer aff002;  Beatriz Bustamante aff003;  Elisa Roncal aff001;  Edgar Neyra aff001;  Patricia Sheen aff001;  Michel Sauvain aff001
Působiště autorů: Laboratorios de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Peru aff001;  Unité Mixte de Recherche 152 Pharmacochimie et Biologie pour le Développement, Institut de Recherche pour le Développement, Université Toulouse III–Paul Sabatier, Toulouse, France aff002;  Clinical Mycology Laboratory, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru aff003
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0218837


The gut microbiota of insects is composed of a wide range of microorganisms which produce bioactive compounds that protect their host from pathogenic attack. In the present study, we isolate and identify the fungus Chrysosporium multifidum from the gut of Hermetia illucens larvae. Extract from C. multifidum culture broth supernatant showed moderate activity against a strain of methicillin-resistant Staphylococcus aureus (MRSA). Bioguided isolation of the extract resulted in the characterization of six α-pyrone derivatives (16) and one diketopiperazine (7). Of these compounds, 5,6-dihydro-4-methoxy-6-(1-oxopentyl)-2H-pyran-2-one (4) showed the greatest activity (IC50 = 11.4 ± 0.7 μg/mL and MIC = 62.5 μg/mL) against MRSA.

Klíčová slova:

Animal sociality – Antimicrobials – Bacterial pathogens – DNA extraction – Fungal pathogens – Fungi – Larvae – Methicillin-resistant Staphylococcus aureus


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