CPO Complete, a novel test for fast, accurate phenotypic detection and classification of carbapenemases

Autoři: Gina K. Thomson aff001;  Sameh AbdelGhani aff002;  Kenneth S. Thomson aff002
Působiště autorů: University of Louisville Hospital, Microbiology Department, Louisville, Kentucky, United States of America aff001;  University of Louisville School of Medicine, Department of Pathology and Laboratory Medicine Louisville, Kentucky, United States of America aff002;  Beni-Suef University School of Pharmacy, Department of Microbiology and Immunology, Beni-Suef, Egypt aff003
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0220586


Carbapenemase-producing organisms (CPOs) are Gram-negative bacteria that are typically resistant to most or all antibiotics and are responsible for a global pandemic of high mortality. Rapid, accurate detection of CPOs and the classification of their carbapenemases are valuable tools for reducing the mortality of the CPO-associated infections, preventing the spread of CPOs, and optimizing use of new β-lactamase inhibitor combinations such as ceftazidime/avibactam, meropenem/vaborbactam and imipenem/relebactam. The current study evaluated the performance of CPO Complete, a novel, manual, phenotypic carbapenemase detection and classification test. The test was evaluated for sensitivity and specificity against 262 CPO isolates of Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii and 67 non-CPO isolates. It was also evaluated for carbapenemase classification accuracy against 205 CPOs that produced a single carbapenemase class. The test exhibited 100% sensitivity 98.5% specificity for carbapenemase detection within 90 minutes and detected 74.1% of carbapenemases within 10 minutes. In the classification evaluation, 99.0% of carbapenemases were correctly classified for isolates that produced a single carbapenemase. The test is technically simple and has potential for adaptation to automated instruments. With lyophilized kit storage at temperatures up to 38°C, the CPO Complete test has the potential to provide rapid, accurate carbapenemase detection and classification in both limited resource and technologically advanced laboratories.

Klíčová slova:

Antibiotic resistance – Antibiotics – Catalogs – Death rates – Enterobacteriaceae – Pseudomonas aeruginosa – Toxic agents


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2019 Číslo 12
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