Translocation of Mycobacterium tuberculosis after experimental ingestion

Autoři: Mustapha Fellag aff001;  Ahmed Loukil aff002;  Jamal Saad aff001;  Hubert Lepidi aff002;  Fériel Bouzid aff002;  Fabienne Brégeon aff002;  Michel Drancourt aff002
Působiště autorů: IHU Méditerranée Infection, Marseille, France aff001;  Aix-Marseille Univ., IRD, MEPHI, IHU Méditerranée Infection, Marseille, France aff002
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article


Human tuberculosis is a life-threatening infection following the inhalation of Mycobacterium tuberculosis, while the closely related bacteria Mycobacterium bovis and Mycobacterium canettii are thought to be transmitted by ingestion. To explore whether M. tuberculosis could also infect individuals by ingestion, male BALBc mice were fed 2 x 106 CFUs of M. tuberculosis Beijing or phosphate-buffered saline as a negative control, over a 28-day experiment. While eight negative control mice remained disease-free, M. tuberculosis was identified in the lymph nodes and lungs of 8/14 mice and in the spleens of 4/14 mice by microscopy, PCR-based detection and culture. Whole-genome sequencing confirmed the identity of the inoculum and the tissue isolates. In these genetically identical mice, the dissemination of M. tuberculosis correlated with the results of the culture detection of four intestinal bacteria. These observations indicate that ingested M. tuberculosis mycobacteria can translocate, notably provoking lymphatic tuberculosis.

Klíčová slova:

DNA extraction – Fluorescent in situ hybridization – Gastrointestinal tract – Lymph nodes – Mouse models – Spleen


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2019 Číslo 12
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