Pathologic changes and immune responses against Coxiella burnetii in mice following infection via non-invasive intratracheal inoculation


Autoři: Xueyuan Hu aff001;  Yonghui Yu aff001;  Junxia Feng aff001;  Mengjiao Fu aff001;  Lupeng Dai aff001;  Zhiyu Lu aff001;  Wenbo Luo aff001;  Jinglin Wang aff001;  Dongsheng Zhou aff001;  Xiaolu Xiong aff001;  Bohai Wen aff001;  Baohua Zhao aff002;  Jun Jiao aff001
Působiště autorů: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, China aff001;  College of Life Sciences, Hebei Normal University, Yuhua District, Shijiazhuang, Hebei, China aff002
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225671

Souhrn

Q fever is a worldwide zoonosis caused by Coxiella burnetii. Human Q fever is typically acquired through inhalation of contaminated aerosols, resulting in an initial pulmonary infection. In this study, BALB/c mice were infected with C. burnetii via an intratracheal (IT) route using a non-invasive aerosol pulmonary delivery device to directly place the living C. burnetii organisms into the lungs of the mice. The bacterial loads, pathological lesions, and antibody and cellular responses were analyzed and compared with those of mice infected via an intraperitoneal (IP) route. Compared with mice infected via an IP route, mice infected via an IT route exhibited a higher bacterial load and more severe pathological lesions in the heart and lungs at days 3 and 7 post-infection (pi). The levels of interferon-γ and IL-12p70 in the serum of mice infected via the IT route were significantly higher than those of mice infected via the IP route at day 3 pi. In conclusion, this murine model of acute C. burnetii infection via IT inoculation closely resembles the natural route of C. burnetii infection than that of IP injection. Thus, this newly developed model will be useful for investigating the pathogenesis and immunity of C. burnetii aerosol infection, as well as for the evaluation of therapeutic drugs and preventive vaccines of Q fever.

Klíčová slova:

Aerosols – Animal models of infection – Coxiella burnetii – Cytokines – Mouse models – Q fever – Spleen


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