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Influence of Debaryomyces hansenii on bacterial lactase gene diversity in intestinal mucosa of mice with antibiotic-associated diarrhea


Autoři: Yunshan He aff001;  Yuan Tang aff001;  Maijiao Peng aff001;  Guozhen Xie aff001;  Wenge Li aff002;  Zhoujin Tan aff001
Působiště autorů: Hunan University of Chinese Medicine, Changsha, Hunan Province, China aff001;  Hunan Institute of Nuclear Agricultural Sciences and Space-induced Breeding, Changsha, Hunan province, China aff002
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0225802

Souhrn

Aim

The current study aimed to investigate the effects of Debaryomyces hansenii on the diversity of bacterial lactase gene in the intestinal mucosa of antibiotic-associated diarrhea (AAD) mice.

Methods

Eighteen mice were randomly divided into three groups (6 mice per group): healthy control group, diarrhea model group and D. hansenii treatment group. The antibiotic-associated diarrhea model was established by intragastric administration with a mixture of cephradine and gentamicin sulfate (23.33 mL·kg-1·d-1) twice a day for 5 days continuously. After establishing the AAD model, the mice in the D. hansenii treatment group were gavaged with D. hansenii for three days, while other groups were gavaged with distilled water. Then, the intestinal mucosa of all three groups was collected and DNA was extracted in an aseptic environment for the following analysis.

Results

The difference in the richness and homogeneity of the bacterial lactase gene among all samples were inapparent, as the difference in the Chao1, ACE, Simpson and Shannon indices among the three groups were insignificant (P>0.05). NMDS analysis also showed that the distance of the samples among the three groups was unobvious. Furthermore, the bacterial lactase gene in the mucosa mainly originated from Actinobacteria, Firmicutes and Proteobacteria. Compared with the healthy control group, the abundance of lactase genes originating from Cupriavidus, Lysobacter, Citrobacter, Enterobacter and Pseudomonas was increased in the D. hansenii treatment group, while the lactase gene from Acidovorax and Stenotrophomonas decreased (p < 0.01 or p < 0.05) in the diarrhea model group and the D. hansenii treatment group.

Conclusion

D. hansenii was capable of improving the growth of some key lactase-producing bacteria like Deinococcus, Cupriavidus and Lysobacter for treating AAD.

Klíčová slova:

Actinobacteria – Antibiotics – Bacteria – Diarrhea – DNA extraction – Gastrointestinal tract – Shannon index – Simpson index


Zdroje

1. Hammer HF, Hammer J. Diarrhea caused by carbohydrate malabsorption. Gastroenterology Clinics of North America. 2012; 41: 611–627. doi: 10.1016/j.gtc.2012.06.003 22917167

2. Zhang YY, Zhou LL, Yang SF. The basic research of primary lactase deficiency. International Journal of Pediatrics. 2014; 41: 302–304. https://doi.org/10.3760/cma.j.issn.1673-4408.2014.03.023

3. Tan ZJ, Guo KX, Zeng A, Guo ZH, Wang H. Advances in research of intestinal lactase. World Chinese Journal of Digestology. 2013; 21: 2897–2901. http://dx.doi.org/10.11569/wcjd.v21.i28.2897

4. Li XQ, Zhou F, Li YC, Guo YQ, Wang XH. Clinical significance of 13C exhalation test on lactose intolerance secondary to rotavirus diarrhea. Journal of Chinese Practical Diagnosis and Therapy. 2011; 25: 399–340.

5. Alexandre V, Even PC, Larue-Achagiotis C, Blouin JM, Blachier F, Benamouzig R, et al. Lactose malabsorption and colonic fermentations alter host metabolism in rats. British Journal of Nutrition. 2013; 110: 625–631. doi: 10.1017/S0007114512005557 23321004

6. Ojetti V, Gigante G, Gabrielli M, Ainora ME, Mannocci A, Lauritano EC, et al. The effect of oral supplementation with lactobacillus reuteri or tilactase in lactose intolerant patients: randomized trial. European Review for Medical & Pharmacological Sciences. 2010; 14: 163–170. https://doi.org/10.1016/j.euroneuro.2009.11.008 20391953

7. Wang W, Yue Y, Hao LL, Yao P, Wei JJ. Clinical effect of the treatment of antibiotic-associated diarrhea. Chinese Journal of Nosocomiology. 2016; 26: 1258–1260. https://doi.org/10.11816/cn.ni.2015-152548

8. Zhu HY. Analysis of the effect of brassella on preventing antibiotic related diarrhea in infants and young children. World Latest Medicine Information. 2017; 17: 59. https://doi.org/10.19613/j.cnki.1671-3141.2017.89.050

9. Zhang X, Guo J. Research progress on association of probiotics with intestinal mucosal immunity. Animal Husbandry and Feed Science. 2017; 38: 58–64. https://doi.org/10.16003/j.cnki.issn1672-5190.2017.11.015

10. Zhai QX, Tian FW, Wang G, Chen W. Progress in research on the role of intestinal microbiota in human health. Food Science. 2013; 34: 337–341. https://doi.org/10.7506/spkx1002-6630-201315069

11. Ding K, Yu ZH, Wang TQ. Progress on gut mucosal immunization. Progress in Veterinary Medicine. 2008; 28: 67–71. https://doi.org/10.3969/j.issn.1007-5038.2007.10.016

12. Li Q, Liu LX. Research progress on the relationship between intestinal mucosal barrier and intestinal endotoxemia. Chinese Journal of Digestion and Medical Imageology(Electronic Editon). 2012; 2: 291–294. https://doi.org/10.7666/d.y418243

13. Zhang L, Qiao M, Liang QH, Chen XQ, Huang LH, Li CX. Protective effect of lactobacillus rhamnosus GG on the damage of gut barrier function in SD rats with antibiotics induced diarrhea. Chinese Journal of Microecology. 2011; 23: 38–43, 48. https://doi.org/10.13381/j.cnki.cjm.2011.01.029

14. Long CX, He L, Guo YF, Liu YW, Xiao NQ, Tan ZJ. Diversity of bacterial lactase genes in intestinal contents of mice with antibiotics-induced diarrhea. World Journal of Gastroenterology. 2017; 23: 7584–7593. doi: 10.3748/wjg.v23.i42.7584 29204058

15. Yang QX, Wang Z. Distribution and function of yeast in eco-environments. Environmental Science & Technology. 2009; 32: 86–91. https://doi.org/10.3969/j.issn.1003-6504.2009.04.021

16. Gadanho M, Almeida JM, Sampaio JP. Assessment of yeast diversity in a marine environment in the south of Portugal by microsatellite-primed PCR. Antonie Van Leeuwenhoek. 2003; 84: 217–227. doi: 10.1023/a:1026038213195 14574117

17. Petersen KM, Westall S, Jespersen L. Microbial succession of Debaryomyces hansenii strains during the production of danish surfaced-ripened cheeses. Journal of Dairy Science. 2002; 85: 478–486. doi: 10.3168/jds.S0022-0302(02)74098-8 11949849

18. Cano-Garcja L, Belloch C, Flores M. Impact of Debaryomyces hansenii strains inoculation on the quality of slow dry-cured fermented sausages. Meat Science. 2014; 96: 1469–1477. doi: 10.1016/j.meatsci.2013.12.011 24423452

19. Xiao XY, Liu YJ, Deng YL, Guo KX, Tan ZJ. Isolation and identification of a yeast strain from intestine of mice. Journal of Hunan Agricultural University. 2016; 42: 419–423. https://doi.org/10.13331/j.cnki.jhau.2016.04.014

20. Prista C, Michán C, Miranda IM. The halotolerant Debaryomyces hansenii, the cinderella of non-conventional yeasts. Yeast. 2016; 33: 523–533. doi: 10.1002/yea.3177 27279567

21. Banjara N, Nickerson KW, Suhr MJ, Nickerson, Hallen-Adamset HE. Killer toxin from several food-derived Debaryomyces hansenii strains effective against pathogenic candida yeasts. International Journal of Food Microbiology. 2016; 222: 23–29. doi: 10.1016/j.ijfoodmicro.2016.01.016 26828815

22. Xiao XY, Liu YJ, Deng YL, Tan ZJ. Tolerance experiment of Debaryomyces hansenii in the environment of gastric acid or intestinal bile salts. Hunan Agricultural Science. 2016; 6: 6–8. https://doi.org/10.16498/j.cnki.hnnykx.2016.06.003

23. Guo KX, Tan ZJ, Xie MZ, Yu Y, Wang XH. The synergic effect of ultra-micro powder qiweibaizhusan combined with yeast on dysbacteriotic diarrhea mice. Chinese Journal of Applied and Environmental Biology. 2015; 21: 61–67. https://doi.org/10.3724/SP.J.1145.2013.10002

24. Long CX, He L, Guo KX, Tan ZJ, Yin KK. Effect of qiweibaizhusan powder combined with yeast on the intestinal bacteria diversity in dysbacteriotic diarrhea mice. Chinese Journal of Integrated Traditional and Western Medicine. 2018; 38: 66–70. https://doi.org/10.7661/j.cjim.20171207.299

25. Liu YJ, Xiao XY, Deng YL, Guo KX, She Y, Tan ZJ. Effects of qiweibaizhusan combined with yeast on intestinal lactobacillus diversity in dysbacteriotic diarrhea mice. Space Medicine and Medical Engineering. 2016; 29: 175–180. https://doi.org/10.16289/j.cnki.1002-0837.2016.03.004

26. Zeng A, Zhang HL, Tan ZJ, Cai Y, Cai GX, Zhou SN. The construction of mice diarrhea model due to dysbacteriosis and curative effect of ultra-micro qiweibaizhusan. Microbiology. 2012; 39: 1341–1348. https://doi.org/10.13344/j.microbiol.china.2012.09.012

27. Jin L, Yang XH, Ren JL, Li JL, Guo XY, Cao P, et al. Effect of dietary compound probiotics on disaccharidase in small intestine mucosa of layer breeders. China Poultry. 2012; 34:14–17. https://doi.org/10.16372/j.issn.1004-6364.2012.12.007

28. Wu H, Zhou SN, Guo C, Tan ZJ, Cai GX, Zeng A, et al. A metagenome DNA extracting method of intestinal flora in mice for molecular diversity analysis based on PCR technology. Chinese Journal of Microecology. 2012; 24: 648–651. https://doi.org/10.13381/j.cnki.cjm.2012.07.003

29. He L, Long CX, Liu YJ, Guo YF, Xiao NQ, Tan ZJ. Effects of Debaryomyces hansenii treatment on intestinal microorganisms in mice with antibiotics-induced diarrhea. 3 Biotech. 2017; 7: 347. doi: 10.1007/s13205-017-0953-9 28955644

30. Long CX, He L, Liu YJ, Hui HY, Tan ZJ, Li DD. Universal primer for analysis of the diversity of intestinal bacterial lactase gene. Chinese Journal of Applied and Environmental Biology. 2017; 23: 758–763. https://doi.org/10.3724/SP.J.1145.2016.10008

31. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. QIIME allows analysis of high-throughput community sequencing data. Nature Methods. 2010; 7: 335–336. doi: 10.1038/nmeth.f.303 20383131

32. Shannon CE. The mathematical theory of communication. 1963. Bell Labs Technical Journal. 1997; 3: 31–32. https://doi.org/10.1063/1.3067010

33. Mahafee WF, Kloepper JW. Temporal changes in the bacterial communities of soil, rhizosphere, and endorhiza associated with field-grown cucumber (Cucumis sativus L). Microbial Ecology. 1997; 34: 210–223. doi: 10.1007/s002489900050 9337416

34. Pitta DW, Pinchak E, Dowd SE, Osterstock J, Gontcharova V, Youn E, et al. Rumen bacterial diversity dynamics associated with changing from bermudagrass hay to grazed winter wheat diets. Microbial Ecology. 2010; 59: 511–512. doi: 10.1007/s00248-009-9609-6 20037795

35. Pitta DW, Parmar N, Patel AK, Indugu N, Kumar S, Prajapathi KB, et al. Bacterial diversity dynamics associated with different diets and different primer pairs in the rumen of Kankrej cattle. PloS One 2014; 9: e111710. doi: 10.1371/journal.pone.0111710 25365522

36. Ramette A. Multivariate analyses in microbial ecology. Fems Microbiology Ecology. 2007; 62: 142–160. doi: 10.1111/j.1574-6941.2007.00375.x 17892477

37. O'Hara AM, Shanahan F. Gut microbiota: mining for therapeutic potential. Clinical Gastroenterology and Hepatology. 2007; 5: 274–284. doi: 10.1016/j.cgh.2006.12.009 17368226

38. Li K, Nie YQ. Research progress on the correlation between intestinal microbial metabolism and drug therapy. Modern Digestion & Intervention. 2017; 22: 756–759. https://doi.org/10.3969/j.issn.1672-2159.2017.05.052

39. Zhang LX, Zhao CP. Antibiotic-associated diarrhea and rational use of drugs. Chinese Community Doctors. 2012; 14: 36–37. https://doi.org/10.3969/j.issn.1007-614x.2012.07.032

40. Ren J, Luo YY. Intestinal microorganism and intestinal diseases. Chinese Journal of Surgery of Integrated Traditional and Western Medicine. 2015; 21: 632–635. https://doi.org/10.3969/j.issn.1007-6948.2015.06.029

41. Ueatrongchit T, Asano Y. Highly selective L-threonine 3-dehydrogenase from Cupriavidus necator and its use in determination of L-threonine. Analytical Biochemistry. 2011; 41: 44–56. https://doi.org/10.1016/j.ab.2010.11.003

42. Liu MJ, Yang S, Cheng KY, Wang LY, Hua YJ. Research progress of deinococcus and Its application. Acta Agriculturae Nucleatae Sinica. 2017; 31: 1723–1729. https://doi.org/10.11869/j.issn.100-8551.2017.09.1723

43. Wang N, Wu KY, Cui LJ, Zhang HW. Advance in bacteria identification and biocontrol mechanism of Lysobacterspp. Journal of Northwest A & F University(Natural Science Edition). 2015; 43: 174–182, 191. https://doi.org/10.13207/j.cnki.jnwafu.2015.05.010

44. Liakopoulos A, van den Bunt G, Geurts Y, Bootsma MCJ, Toleman M, Ceccarelli D, et al. High prevalence of intra-familial co-colonization by extended-spectrum cephalosporin resistant enterobacteriaceae in preschool children and their parents in dutch households. Frontiers in Aicrobiology. 2018; 9: 293. doi: 10.3389/fmicb.2018.00293 29515562

45. Ghanavati R, Emaneini M, Kalantar-Neyestanaki D, Maraji AS, Dalvand M, Beigverdi R, et al. Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase and AmpC β-lactamase-producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran. Revista da Sociedade Brasileira de Medicina Tropical. 2018; 51, 88–93. doi: 10.1590/0037-8682-0227-2017 29513851

46. Guo QP. Study on the regulation of small intestinal mucosa binding proteins, cytokines, and related toll-like receptors by probiotics enterococcus aureus and pyrethrocytes. Guangxi University. 2017.

47. Palleja A, Mikkelsen KH, Forslund SK, Kashani A, Allin KH, Nielsen T, et al. Recovery of gut microbiota of healthy adults following antibiotic exposure. Nature Microbiology. 2018; 3: 1255–1265. doi: 10.1038/s41564-018-0257-9 30349083

48. Liu LY, Xie P. Effects of microcystotoxin-LR on the digestive enzymes activity of intestinal tract in Balc/c mice. Acta Hydrobiologica Sinica. 2014; 38: 333–539. https://doi.org/10.7541/2014.75

49. Wang K, Ma ZM, Chang C, Wang XD, Wu JE. Effect of Saccharomyces boulardii on intestinal enzyme activity of meat ducks with intestinal dysbacteriosis. Hubei Agricultural Science. 2015; 54: 2690–2694. https://doi.org/10.14088/j.cnki.issn0439-8114.2015.11.035.


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